Mikrogen Diagnostik recomLine Yersinia IgA (IgM) 2.0 Lateral Strip Test Kit (4673)

$440.00

Host Quantity Applications Species Reactivity Data Sheet  
20 Tests Lateral Strip Assay Yersinia
SKU: 4673 Categories: ,
Overview
Product Name Mikrogen Diagnostik recomLine Yersinia IgA (IgM) 2.0 Lateral Strip Test Kit (4673)
Description Strip-Immunoassay with antigens produced by recombinant techniques for the detection of IgG, IgM or IgA antibodies directed Y. enterocolitica and Y. pseudotuberculosis. Detection of species-specific IgG antibodies makes it possible to differentiate between Y. enterocolitica and Y. pseudotuberculosis.

Product is intended for research use.
Target Human Yersinia IgA (IgM)
Species Reactivity Yersinia
Assay Type Indirect Sandwich ELISA
Applications Lateral Strip Assay
Properties
Background The enteropathogenic Yersinia species, Yersinia enterocolitica and Yersinia pseudotuberculosis, have a global distribution and have become increasingly important in recent years. These pathogens are transmitted orally either in food (especially meat) or in contaminated water. Typical symptoms of an acute Y. enterocolitica infection are watery, sometimes bloody diarrhoea with abdominal pain, vomiting and fever. With Y. pseudotuberculosis infection, mesenteric lymphadenitis with terminal ileitis can be observed clinically. Since it is difficult to distinguish this clinical picture from appendicitis, it is also referred to as "pseudoappendicitisâ€. Post infectious complications such as reactive arthritis, erythema nodosum and other rheumatic diseases can occur, especially with HLA-B27 carriers. High and persistent IgA titres against Yersinia antigens are characteristic of these patients.For the recomLine Yersinia tests, plasmid-encoded virulence proteins localised on the cell surface (Yersinia outer proteins) and adhesins are produced recombinantly in order to differentiate serologically between the species Y. enterocolitica and Y. pseudotuberculosis. These proteins are only expressed by Yersinia strains that are pathogenic to humans. The recomLine Yersinia tests make it possible to detect past Yersinia infections, and are thus ideally suited for identification of Yersinia-induced immunopathological complications and chronic yersiniosis. Detection of IgG and IgA antibodies can be a very useful diagnostic tool if Yersinia-induced arthritis is suspected.
Sample Type Serum, plasma, whole blood
Assay Resolution See Figure
Components
10X Wash Buffer100 mL
TMB Substrate40 mL
Milk Powder5 g
Instuctions for Use1 Each
Evaluation Form1 Each
Test Strips2 Vials x 10 Each
Anti-Human IgA Conjugate500 uL
Specificity Information
Target ID Human Yersinia IgA (IgM)
Research Areas Infectious Disease
Application Images
Description Strip layout.
Description Test principle and procedure:

1. 1st Incubation. A test strip loaded with Yersinia antigens is incubated with diluted serum or plasma in a dish for 1 hour.
2. Wash 3 times
3. 2nd Incubation
4. Peroxidase conjugated anti-human antibodies (IgG, IgA or IgM specific) are added. Incubate for 45 minutes.
5. Wash 3 times
6. Color reaction
7. 8 minutes after addition of the coloring solution, insoluble colored bands develop at the sites on the test strips occupied by antibodies.
Description Antigens used.
Description Diagnostic sensitivity.
Description Diagnostic specificity.
Description Differentiation between Y. enterocolitica and Y. pseudotuberculosis by detecting species-specific IgG antibodies.
* Classified as Y. enterocolitica samples with a positive Widal test result
** Classified as Y. pseudotuberculosis samples with a positive culture and PCR results
Additional Information
Handling
Storage Store at 2-8°C.
References & Data Sheet