qed-bioscience-recomline-tropical-fever-igm-lateral-strip-test-kit-7873-1

qed-bioscience-recomline-tropical-fever-igm-lateral-strip-test-kit-7873-2

Mikrogen Diagnostik recomLine Tropical Fever IgM Lateral Strip Test Kit (7873)

$929.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
	20 Tests	Lateral Strip Assay	Tropical Fever

SKU: 7873 Categories: Infectious Disease Test Kits and Positive Controls, MIKROGEN Products

Overview

Product Name Mikrogen Diagnostik recomLine Tropical Fever IgM Lateral Strip Test Kit (7873)

Description The first global immunoblot assay for simultaneous detection and differentiation of Dengue (DENV), Chikungunya (CHIKV) and Zika virus (ZIKV) infections, CE marked, Patent Pending

Product is intended for research use.

Target Human Tropical Fever IgM

Species Reactivity Tropical Fever

Assay Type Indirect Sandwich ELISA

Applications Lateral Strip Assay

Associated Products recomLine Tropical Fever IgG Lateral Strip Test Kit (7872)

Properties

Background Dengue, Chikungunya and Zika viruses are primarily transmitted by mosquitoes from the Aedes genus. Dengue is widespread in tropical and subtropical regions around the world and is one of the most common viral diseases transmitted by mosquitoes with an estimated 390 million infections each year.Since the circulation areas of DENV, ZIKV and CHIKV partly overlap and the pathogens induce similar clinical symptoms in the early stages, reliable differential diagnosis is crucial. While CHIKV belongs to the family of togaviruses / genus alphavirus, DENV and ZIKV are flaviviruses /genus flavivirus. The close genetic relationship between the flaviviruses makes serological differentiation difficult especially in the widespread Dengue endemic areas. For secondary flavivirus infections it has to be considered that the IgM response may be low or even undetectable. In parallel, there can be a significant IgG increase specific for the primary flavivirus infection before or in parallel to (IgM)/IgG seroconversion of the secondary flavivirus infection.Serological evaluation should always be interpreted with respect to IgG and IgM test results, the time of infection (date of sampling after onset of clinical symptoms), and the whereabouts of the patient over the 6 weeks prior to onset of disease. Current Dengue assays usually rely on the detection of antibodies to the viral envelope (E) protein, which is known to be very sensitive but also highly cross-reactive with antibodies against the E protein of other flaviviruses such as Zika virus (ZIKV), Yellow Fever virus (YFV), West Nile virus (WNV), Tick-borne Encephalitis virus (TBEV), and Japanese Encephalitis virus (JEV). Meanwhile, variants of the E protein, so call Equad proteins have been described, which have a higher specificity due to targeted mutations. Furthermore, the serological detection of antibodies against the Non-structural protein 1 (NS1) has been reputed to have diagnostic value due to minimal cross-reactivity among NS1 proteins from different flaviviruses.recomLine Tropical Fever IgG, IgM immunoblot combines those diagnostic markers and enables the identification of specific antibodies against single antigens from Dengue, Chikungunya and Zika viruses. Virus-like particles (VLP) are used to detect CHIKV and for DENV and ZIKV NS1 and Equad proteins are applied. The unique setup allows the differentiation of DENV and ZIKV by an interpretation scheme in two steps, which takes the NS1 antigen reactivities and in their absence the Equad antigen reactivities into account.

Sample Type Serum, plasma, whole blood

Assay Resolution See Figure

Components

10X Wash Buffer	100 mL
TMB Substrate	40 mL
Milk Powder	5 g
Instuctions for Use	1 Each
Evaluation Form	1 Each
Test Strips	2 Vials x 10 Each
Anti-Human IgM Conjugate	500 uL

Target ID Human Tropical Fever IgM

Research Areas Infectious Disease

Description Strip layout.

Description Test principle and procedure:

1. 1st Incubation. A test strip loaded with DENV, CHIKV and ZIKV antigens is incubated with diluted serum or plasma in a dish for 1 hour.
2. Wash 3 times
3. 2nd Incubation
4. Peroxidase conjugated anti-human antibodies (IgG or IgM specific) are added. Incubate for 45 minutes.
5. Wash 3 times
6. Color reaction. 8 minutes after addition of the coloring solution, insoluble colored bands develop at the sites on the test strips occupied by antibodies.