Mikrogen Diagnostik recomLine Tropical Fever IgG Lateral Strip Test Kit (7872)
$929.00
Host | Quantity | Applications | Species Reactivity | Data Sheet | |
---|---|---|---|---|---|
20 Tests | Lateral Strip Assay | Tropical Fever |
SKU: 7872
Categories: Infectious Disease Test Kits and Positive Controls, MIKROGEN Products
Overview
Product Name Mikrogen Diagnostik recomLine Tropical Fever IgG Lateral Strip Test Kit (7872)
Description The first global immunoblot assay for simultaneous detection and differentiation of Dengue (DENV), Chikungunya (CHIKV) and Zika virus (ZIKV) infections, CE marked, Patent Pending
Product is intended for research use.
Product is intended for research use.
Target Human Tropical Fever IgG
Species Reactivity Tropical Fever
Assay Type Indirect Sandwich ELISA
Applications Lateral Strip Assay
Associated Products recomLine Tropical Fever IgM Lateral Strip Test Kit (7873)
Properties
Background Dengue, Chikungunya and Zika viruses are primarily transmitted by mosquitoes from the Aedes genus. Dengue is widespread in tropical and subtropical regions around the world and is one of the most common viral diseases transmitted by mosquitoes with an estimated 390 million infections each year.Since the circulation areas of DENV, ZIKV and CHIKV partly overlap and the pathogens induce similar clinical symptoms in the early stages, reliable differential diagnosis is crucial. While CHIKV belongs to the family of togaviruses / genus alphavirus, DENV and ZIKV are flaviviruses /genus flavivirus. The close genetic relationship between the flaviviruses makes serological differentiation difficult especially in the widespread Dengue endemic areas. For secondary flavivirus infections it has to be considered that the IgM response may be low or even undetectable. In parallel, there can be a significant IgG increase specific for the primary flavivirus infection before or in parallel to (IgM)/IgG seroconversion of the secondary flavivirus infection.Serological evaluation should always be interpreted with respect to IgG and IgM test results, the time of infection (date of sampling after onset of clinical symptoms), and the whereabouts of the patient over the 6 weeks prior to onset of disease. Current Dengue assays usually rely on the detection of antibodies to the viral envelope (E) protein, which is known to be very sensitive but also highly cross-reactive with antibodies against the E protein of other flaviviruses such as Zika virus (ZIKV), Yellow Fever virus (YFV), West Nile virus (WNV), Tick-borne Encephalitis virus (TBEV), and Japanese Encephalitis virus (JEV). Meanwhile, variants of the E protein, so call Equad proteins have been described, which have a higher specificity due to targeted mutations. Furthermore, the serological detection of antibodies against the Non-structural protein 1 (NS1) has been reputed to have diagnostic value due to minimal cross-reactivity among NS1 proteins from different flaviviruses.recomLine Tropical Fever IgG, IgM immunoblot combines those diagnostic markers and enables the identification of specific antibodies against single antigens from Dengue, Chikungunya and Zika viruses. Virus-like particles (VLP) are used to detect CHIKV and for DENV and ZIKV NS1 and Equad proteins are applied. The unique setup allows the differentiation of DENV and ZIKV by an interpretation scheme in two steps, which takes the NS1 antigen reactivities and in their absence the Equad antigen reactivities into account.
Sample Type Serum, plasma, whole blood
Assay Resolution See Figure
Components
10X Wash Buffer | 100 mL |
TMB Substrate | 40 mL |
Milk Powder | 5 g |
Instuctions for Use | 1 Each |
Evaluation Form | 1 Each |
Test Strips | 2 Vials x 10 Each |
Anti-Human IgG Conjugate | 500 uL |
Specificity Information
Target ID Human Tropical Fever IgG
Research Areas Infectious Disease
Application Images
Description Strip layout.
Description Test principle and procedure:
1. 1st Incubation. A test strip loaded with DENV, CHIKV and ZIKV antigens is incubated with diluted serum or plasma in a dish for 1 hour.
2. Wash 3 times
3. 2nd Incubation
4. Peroxidase conjugated anti-human antibodies (IgG or IgM specific) are added. Incubate for 45 minutes.
5. Wash 3 times
6. Color reaction. 8 minutes after addition of the coloring solution, insoluble colored bands develop at the sites on the test strips occupied by antibodies.
1. 1st Incubation. A test strip loaded with DENV, CHIKV and ZIKV antigens is incubated with diluted serum or plasma in a dish for 1 hour.
2. Wash 3 times
3. 2nd Incubation
4. Peroxidase conjugated anti-human antibodies (IgG or IgM specific) are added. Incubate for 45 minutes.
5. Wash 3 times
6. Color reaction. 8 minutes after addition of the coloring solution, insoluble colored bands develop at the sites on the test strips occupied by antibodies.
Description Test recombinant antigens.
Description Sensitivity
Description Specificity
Additional Information
Additional Information Additional Mirkogen Kit Information
Handling
Storage Store at 2-8°C.
References & Data Sheet
Data Sheet Download PDF Data Sheet