Mikrogen Diagnostik recomWell Treponema IgM ELISA Kit (5105)

$393.00

Host Quantity Applications Species Reactivity Data Sheet  
96 Tests ELISA Treponema
SKU: 5105 Categories: ,
Overview
Product Name Mikrogen Diagnostik recomWell Treponema IgM ELISA Kit (5105)
Description Enzyme immunoassay with antigens produced by recombinant techniques for the detection of IgG and IgM antibodies against Treponema pallidum

Product is intended for research use.
Target Human Treponema IgM
Species Reactivity Treponema
Assay Type Indirect ELISA
Applications ELISA
Properties
Background Treponema pallidum subsp. pallidum is the causative agent of Syphilis, a chronic disease occuring only in humans. The disease progresses through stages. After a short propagation period at the site of infection (primary stage) the hematogenic spreading of the pathogen throughout the entire organism follows (secondary stage). After the decline of acute symptoms Treponema pallidum can persist for years or lifetime in the human organism (stage of latency) and then can cause late complications (tertiary Syphilis). During pregnancy the pathogen can be transmitted to the fetus (Syphilis connata). In approximately 40 % of the cases infection results in abortion or death of the child shortly after birth. The propability of infection in utero is around 40-70 %.The serodiagnosis of syphilis is performed in steps. As a rule, the T. pallidum hemagglutination assay (TPHA) is used as an antibody screening test. As modern screening test system, the recomWell Treponema IgG, IgM is an excellent alternative. The main advantages of the recomWell Treponema tests are the possibility to distinguish between IgG and IgM antibodies and the capacity to process large numbers of samples accompanied by an automated readout. In addition, by the use of purified, recombinant antigens, the recomWell Treponema tests offer a reproducible higher specificity with maximum sensitivity.
Sample Type Serum, plasma, whole blood
Assay Resolution See Figure
Components
10X Wash Buffer100 mL
Dilution Buffer125 mL
TMB Substrate12 mL
Stop Slution12 mL
Instuctions for Use1 Each
Evaluation Form1 Each
Microplate Sealing Tape2 Each
Microtiter Plate12 x 8 Wells
Positive Control450 uL
Cut-Off Control450 uL
Negative Control450 uL
Anti-Human IgG Conjugate500 uL
Specificity Information
Target ID Human Treponema IgM
Research Areas Infectious Disease
Application Images
Description Test principle and procedure.

Indirect sandwich test. Recombinant antigens are bound to the solid phase.

1. 1st Incubation
2. Add patient samples diluted 1:101 (sample: 10 µl of serum or plasma), incubate for 1 h at 37 °C.
3. Wash 4 times
4. 2nd Incubation
5. Add peroxidase conjugated anti-human IgG or IgM antibodies (conjugate), incubate for 30 min at 37 °C.
6. Wash 4 times
7. Color reaction. Add ready-to-use TMB solution and incubate for 30 min at room temperature. Stop the substrate reaction with H3PO4 and measure the extinction at 450 nm.
Description Test recombinant antigens.

1) Norris SJ and the Treponema Pallidum Polypeptide Research Group Polypeptides of Treponema pallidum: Progress toward Understanding Their Structural, Functional, and Immunologic Roles. Microbiol. Rev. 1993, 57(3), 750-79
2) Chamberlain NR, Brandt ME, Erwin AL, Radolf, JD and Norgard MV Major integral membrane protein immunogens of Treponema pallidum are proteolipids. Infect. Immun. 1989, 57, 2872-7
3) Atkins DR, Purcell BK, Mitra MM, Norgard MV and Radolf JD Lipid modification of the 17-kilodalton membrane immunogen of Treponema pallidum determines macrophage activation as well as amphilicity. Infect. Immun. 1993, 61, 1202-10
Description Sensitivity
Description Specificity
Additional Information
Handling
Storage Store at 2-8°C.
References & Data Sheet