Treponema IgM/recomLine

recomLine Treponema IgG 

recomLine Treponema IgM 

Strip-Immunoassay with antigens produced by recombinant techniques for the detection of IgG or IgM antibodies against Treponema pallidum

Treponema pallidum subsp. pallidum is the causative agent of syphilis, a chronic disease that occurs only in humans. The disease progresses in several stages. After a short propagation period at the site of infection (primary state), the pathogen spreads systemically (secondary state). After the decline of acute symptoms, Treponema pallidum can persist for years (latency stage) and cause later complications (tertiary syphilis and neurosyphilis). 

The recomLine Treponema IgG/IgM assay uses T. pallidum-specific recombinant antigens Tp47, Tp17, Tp15, TmpA, Tp257 (Gpd), and Tp453 and provides an easy and a reliable assay for T. pallidum IgG and IgM antibody. 

• Includes 6 serologically relevant T. pallidum antigens. 

• Separate detection of IgG and IgM antibodies is possible. 

• Control band on every strip. 

Product #5172 recomLine Treponema IgG (reagents for 20 tests) 

Product #5173 recomline Treponema IgM 

(reagents for 20 tests) 

For more information visit https://www.mikrogen.de/english/nc/products/product-overview/testsystem/treponema-igg-1.html 

Available in the U.S. For Research Use Only. 

Not intended for diagnostic procedures.

Test Principle and Procedure 

Recombinant Treponema Antigens 

1st Incubation A test strip loaded with HIV antigens is incubated with diluted sample in a dish for 1-3 hours. 

Wash strip 3 times. 

2nd Incubation Peroxidase conjugated anti-human IgG or IgM is added. Incubate for 45 minutes. 

Wash strip 3 times. 

Color reaction 8 minutes after addition of the coloring solution, insoluble colored bands develop at the sites on the test strips occupied by antibodies. 

Antigen Function Size (kDa) 

Tp47 Membrane protein 47 

TmpA Membrane protein 42 

Tp257 (Gpd) Glycerophosphodiester-Phosphodiesterase, membrane protein 39 

Tp453 Membrane protein 28 

Tp17 Membrane protein 17 

Tp15 Membrane protein 15 

Storage 

+2°C – +8°C 

Product #5172 recomLine Treponema IgG 

(reagents for 20 tests) 

Product #5173 recomLine Treponema IgM 

(reagents for 20 tests)