qed-bioscience-treponema-igg-recomline-lateral-strip-test-kit-5172-1

Mikrogen Diagnostik Treponema IgG/recomLine Lateral Strip Test Kit (5172)

$577.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
	20 Tests	Lateral Strip Assay	Treponema

SKU: 5172 Categories: Infectious Disease Test Kits and Positive Controls, MIKROGEN Products

Overview

Product Name Mikrogen Diagnostik Treponema IgG/recomLine Lateral Strip Test Kit (5172)

Description Strip-Immunoassay with antigens produced by recombinant techniques for the detection of IgG or IgM antibodies against Treponema pallidum

Product is intended for research use.

Target Human Treponema IgG

Species Reactivity Treponema

Assay Type Indirect Sandwich ELISA

Applications Lateral Strip Assay

Associated Products recomWell Treponema IgG ELISA Kit (5104)
recomWell Treponema IgM ELISA Kit (5105)
Treponema IgM/recomLine Lateral Strip Test Kit (5173)

Properties

Background Treponema pallidum subsp. pallidum is the causative agent of Syphilis, a chronic disease occuring only in humans. The disease progresses in several stages. After a short propagation period at the site of infection (primary stage) the hematogenic spreading of the pathogen throughout the entire organism follows (secondary stage). After the decline of acute symptoms Treponema pallidum can persist for years or lifetime in the human organism (stage of latency) and then can cause late complications (tertiary Syphilis and neurosyphilis). During pregnancy the pathogen can be transmitted to the fetus (Syphilis connata). An untreated syphilis can decisively affect the course of pregnancy and can lead to spontaneous abortion, stillborn or premature birth.The serologic diagnosis of syphilis intends the use of a sensitive antibody screening assay (TPHA/TPPA, ELISA) in the first step. In the case of equivocal or positive results in the screening test a confirmation assay is performed in the second step to assure the specificity (FTA-ABS, immunoblot). Here the immunoblot plays a very important role, as only with this technique the determination of antibody production against essential Treponema pallidum antigens is possible. At the same time the detection of different antibody classes is possible. With the use of recombinant antigens the recomLine Treponema IgG, IgM meets all these requirements perfectly.

Sample Type Serum, plasma, whole blood

Assay Resolution See Figure

Components

10X Wash Buffer	100 mL
TMB Substrate	40 mL
Milk Powder	5 g
Instuctions for Use	1 Each
Evaluation Form	1 Each
Test Strips	2 Vials x 10 Each
Anti-Human IgG Conjugate	500 uL

Target ID Human Treponema IgG

Research Areas Infectious Disease

Description Strip layout.

Description Test principle and procedure.

1. 1st Incubation. A test strip loaded with Treponema antigens is incubated with diluted serum or plasma in a dish for 1 hour (3 hours as an alternative).
2. Wash 3 times
3. 2nd Incubation
4. Peroxidase conjugated anti-human antibodies (IgG or IgM specific) are added. Incubate for 45 minutes.
5. Wash 3 times
6. Color reaction. 8 minutes after addition of the coloring solution, insoluble colored bands develop at the sites on the test strips occupied by antibodies.