Mikrogen Diagnostik Treponema IgG/recomLine Lateral Strip Test Kit (5172)
$577.00
SKU: 5172
Categories: Infectious Disease Test Kits and Positive Controls, MIKROGEN Products
Overview
Product Name Mikrogen Diagnostik Treponema IgG/recomLine Lateral Strip Test Kit (5172)
Description Strip-Immunoassay with antigens produced by recombinant techniques for the detection of IgG or IgM antibodies against Treponema pallidum
Product is intended for research use.
Product is intended for research use.
Target Human Treponema IgG
Species Reactivity Treponema
Assay Type Indirect Sandwich ELISA
Applications Lateral Strip Assay
Properties
Background Treponema pallidum subsp. pallidum is the causative agent of Syphilis, a chronic disease occuring only in humans. The disease progresses in several stages. After a short propagation period at the site of infection (primary stage) the hematogenic spreading of the pathogen throughout the entire organism follows (secondary stage). After the decline of acute symptoms Treponema pallidum can persist for years or lifetime in the human organism (stage of latency) and then can cause late complications (tertiary Syphilis and neurosyphilis). During pregnancy the pathogen can be transmitted to the fetus (Syphilis connata). An untreated syphilis can decisively affect the course of pregnancy and can lead to spontaneous abortion, stillborn or premature birth.The serologic diagnosis of syphilis intends the use of a sensitive antibody screening assay (TPHA/TPPA, ELISA) in the first step. In the case of equivocal or positive results in the screening test a confirmation assay is performed in the second step to assure the specificity (FTA-ABS, immunoblot). Here the immunoblot plays a very important role, as only with this technique the determination of antibody production against essential Treponema pallidum antigens is possible. At the same time the detection of different antibody classes is possible. With the use of recombinant antigens the recomLine Treponema IgG, IgM meets all these requirements perfectly.
Sample Type Serum, plasma, whole blood
Assay Resolution See Figure
Components
10X Wash Buffer | 100 mL |
TMB Substrate | 40 mL |
Milk Powder | 5 g |
Instuctions for Use | 1 Each |
Evaluation Form | 1 Each |
Test Strips | 2 Vials x 10 Each |
Anti-Human IgG Conjugate | 500 uL |
Specificity Information
Target ID Human Treponema IgG
Research Areas Infectious Disease
Application Images
Description Strip layout.
Description Test principle and procedure.
1. 1st Incubation. A test strip loaded with Treponema antigens is incubated with diluted serum or plasma in a dish for 1 hour (3 hours as an alternative).
2. Wash 3 times
3. 2nd Incubation
4. Peroxidase conjugated anti-human antibodies (IgG or IgM specific) are added. Incubate for 45 minutes.
5. Wash 3 times
6. Color reaction. 8 minutes after addition of the coloring solution, insoluble colored bands develop at the sites on the test strips occupied by antibodies.
1. 1st Incubation. A test strip loaded with Treponema antigens is incubated with diluted serum or plasma in a dish for 1 hour (3 hours as an alternative).
2. Wash 3 times
3. 2nd Incubation
4. Peroxidase conjugated anti-human antibodies (IgG or IgM specific) are added. Incubate for 45 minutes.
5. Wash 3 times
6. Color reaction. 8 minutes after addition of the coloring solution, insoluble colored bands develop at the sites on the test strips occupied by antibodies.
Description Recombinant Treponema Antigens
Description Diagnostic sensitivity.
Description Diagnostic specificity.
Additional Information
Additional Information Additional Mirkogen Kit Information
Handling
Storage Store at 2 - 8°C. Do not Freeze.
References & Data Sheet
Data Sheet Download PDF Data Sheet