Anti-Streptococcus pneumoniae Antibody (22001)
$624.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Mouse | 200ug | ELISA,WB,In-Vivo Protection | Streptococcus Pneumoniae |  |
Overview
Product Name Anti-Streptococcus pneumoniae Antibody (22001)
Description Anti-Streptococcus pneumoniae Mouse Monoclonal Antibody
Target Streptococcus pneumoniae
Species Reactivity Streptococcus Pneumoniae
Applications ELISA,WB,In-Vivo Protection
Host Mouse
Clonality Monoclonal
Clone ID XI64
Isotype IgM
Immunogen Antigen for 22001 and 22002: heat-killed S. pneumoniae strain R36A.
Associated Products
Associated Antibody Clones
Streptococcus pneumoniae
Capsular |
No. tested |
MAb #22001 |
MAb #22002 |
3 |
10 |
5 |
5 |
4 |
11 |
1 |
4 |
6 |
22 |
4 |
7 |
7 |
1 |
0 |
0 |
9 |
3 |
0 |
3 |
14 |
3 |
0 |
0 |
16 |
1 |
0 |
0 |
19 |
3 |
0 |
0 |
23 |
3 |
1 |
1 |
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity These antibodies recognize pneumococcal surface protein A.
Target ID Streptococcus pneumoniae
Research Areas Infectious Disease
Application Images
Description Crain MJ et al. 1990 Pneumococcal surface protein A (PspA) is serologically highly variable and is expressed by all clinically important capsular s of Streptococcus pneumoniae. Infection and Immunity 58: 3293-3299.
Description Immunoblot of recombinant pneumococcal surface protein A (PspA) reacted with #22002. Lanes: 1 and 2, phage lysate from full-length PspA+ clone with and without induction by isopropyl-β-D-thiogalactopyranoside (IPTG), respectively; 3, cell wall extract from pneumococcal type 2 strain D39. See reference 4 below.
Handling
Storage These antibodies are stable for at least one (1) year from date of receipt at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions ELISA:Catalog No., Titer22001, 1: 16,00022002, 1: 64,00022004, 1: 32,000 ELISA is an indirect assay against Strep. pneumoniae cells on the solid phase. Titrations start with antibodies at 1mg/ml.In vivo protection: These antibodies protect against lethal pneumococcal infection in a mouse model.
References & Data Sheet
References McDaniel L. et al. 1984 Monoclonal antibodies against protease-sensitive pneumococcal antigens can protect mice from fatal infection with Streptococcus pneumoniae. J Exp Med 160: 386-397.
McDaniel L. et al. 1987 A protective monoclonal antibody that reacts with a novel antigen of pneumococcal teichoic acid. Microbial Pathogenesis 3: 249-260.
Crain MJ et al. 1990 Pneumococcal surface protein A (PspA) is serologically highly variable and is expressed by all clinically important capsular s of Streptococcus pneumoniae. Infection and Immunity 58: 3293-3299.
McDaniel L. et al. 1991 PspA, A surface protein of Streptococcus pneumoniae, is capable of eliciting protection against Pneumococci of more than one capsular type. Infection and Immunity 59: 222-228.
McDaniel L. et al. 1987 A protective monoclonal antibody that reacts with a novel antigen of pneumococcal teichoic acid. Microbial Pathogenesis 3: 249-260.
Crain MJ et al. 1990 Pneumococcal surface protein A (PspA) is serologically highly variable and is expressed by all clinically important capsular s of Streptococcus pneumoniae. Infection and Immunity 58: 3293-3299.
McDaniel L. et al. 1991 PspA, A surface protein of Streptococcus pneumoniae, is capable of eliciting protection against Pneumococci of more than one capsular type. Infection and Immunity 59: 222-228.
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet