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CCL2 (50186P-20)

$159.00

Host Quantity Applications Species Reactivity Data Sheet  
20ug Migration assay Human
SKU: 50186P-20 Categories: ,
Overview
Product Name CCL2 (50186P-20)
Description Recombinant human CCL2 is produced in E. coli
Target CCL2
Species Reactivity Human
Applications Migration assay
Source Recombinant human CCL2 is produced in E. coli
Properties
Form Lyophilized
Molecular Mass 8.662 kDa
Purity >97% by SDS-PAGE
Background CCL2, also known as Monocyte Chemoattractant Protein-1 (MCP-1) is a small cytokine (~13kDa) that belongs to the CC chemokine family. CCL2 recruits monocytes, memory T cells, and dendritic cells to sites of inflammation produced by either tissue injury or infection. It is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. To become activated, CCL2 protein has to be cleaved by metalloproteinase MMP-12. CCL2 is implicated in the pathogenesis of several diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis and atherosclerosis. CCR2 and CCR4 are two cell surface receptors that bind CCL2.
Specificity Information
Target Name C-C motif chemokine 2
Target ID CCL2
Alternative Names rHuCCL2, MCP-1
Sequence Location Secreted
Sequence QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAK EICADPKQKWVQDSIDHLDKQTQTPKT
Biological Activity CCL2
Biological Function Acts as a ligand for C-C chemokine receptor CCR2 (PubMed:9837883, PubMed:10587439, PubMed:10529171). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:9837883, PubMed:10587439). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed:8627182, PubMed:9792674, PubMed:8195247). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed:8107690). {PubMed:10529171, PubMed:10587439, PubMed:8107690, PubMed:8195247, PubMed:8627182, PubMed:9792674, PubMed:9837883}.
Background CCL2, also known as Monocyte Chemoattractant Protein-1 (MCP-1) is a small cytokine (~13kDa) that belongs to the CC chemokine family. CCL2 recruits monocytes, memory T cells, and dendritic cells to sites of inflammation produced by either tissue injury or infection. It is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. To become activated, CCL2 protein has to be cleaved by metalloproteinase MMP-12. CCL2 is implicated in the pathogenesis of several diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis and atherosclerosis. CCR2 and CCR4 are two cell surface receptors that bind CCL2.
Application Images
Additional Information
Additional Information Endotoxin Level: <0.01 EU per 1ug of protein by LAL method.Migration or recombinant CCR2- expressing cells.
Handling
Storage 12 months from date of receipt, -20°C to -70°C, as supplied. 1 month,- 20°C to -70°C, under sterile conditions after reconstitution. Best if used immediately after reconstitution. Avoid multiple freeze-thaw cycles.
Dilution Instructions Centrifuge tube prior to resuspending. Recommended at 100ug/ml in sterile distilled water.
References & Data Sheet