Anti-Platelet GMP-140 (P-selectin/CD62) Antibody (50122)

$586.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,IP,FACS,FC Human
SKU: 50122 Categories: , ,
Overview
Product Name Anti-Platelet GMP-140 (P-selectin/CD62) Antibody (50122)
Description Anti-Human Platelet GMP-140 (P-selectin/CD62) Mouse Monoclonal Antibody
Target Platelet GMP-140 (P-selectin/CD62)
Species Reactivity Human
Applications WB,IHC,IP,FACS,FC
Host Mouse
Clonality Monoclonal
Clone ID WGA-1
Isotype IgG1
Immunogen Human platelets
Properties
Form Lyophilized
Formulation PBS, pH 7.4, 1% BSA, lyophilized.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Protein Stabilizer 1% Bovine Serum Albumin
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity This antibody reacts with GMP-140 (P- selectin) on activated human platelets; it does not react with resting platelets. It does not interfere with platelet aggregation or platelet adhesion. It does not cross-react with rabbit, rat, porcine, or canine activated platelets.
Target Name P-selectin
Target ID Platelet GMP-140 (P-selectin/CD62)
Uniprot ID P16109
Alternative Names CD62 antigen-like family member P, Granule membrane protein 140, GMP-140, Leukocyte-endothelial cell adhesion molecule 3, LECAM3, Platelet activation dependent granule-external membrane protein, PADGEM, CD antigen CD62P
Gene Name SELP
Sequence Location Cell membrane, Single-pass type I membrane protein
Biological Function Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand rPubMed:11081633, PubMed:28011641, PubMed:7585950}.
Research Areas Cell adhesion
Application Images
Description Flow cytometry: 1-10ug/ml Thrombin-activated human platelets incubated 30 minutes at room temperature with 1-2ug #50122 per 107 cells/ml and FITC-labeled secondary antibody.
Description Western Blot: 5-10ug/ml, non-reducing and non-heating conditions. Immunohistochemistry: 5-10ug/ml, frozen tissue sections.
Additional Information
Handling
Storage This product does not contain preservative. Lyophilized antibody is stable at 4°C for 2 years. Store the reconstituted solution (2. 0mg/ml) in aliquots at -20°C for one year or at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.
Dilution Instructions Reconstitute lyophilized antibody in 50ul of distilled water (2.0mg/ml final concentration).
Application Instructions ELISA: 1-10ug/ml with immobilized antigen.Flow cytometry: 1-10ug/mlWestern Blot: 5-10ug/ml, non-reducing and non-heating conditions.

Immunohistochemistry: 5-10ug/ml, frozen tissue sections.
References & Data Sheet