Mikrogen Diagnostik recomLine Parvovirus B19 IgM Lateral Strip Test Kit (4473)

$550.00

Host Quantity Applications Species Reactivity Data Sheet  
20 Tests Lateral Strip Assay Parvovirus B19
SKU: 4473 Categories: ,
Overview
Product Name Mikrogen Diagnostik recomLine Parvovirus B19 IgM Lateral Strip Test Kit (4473)
Description Strip-Immunoassay with antigens produced by recombinant techniques for the detection of IgG and IgM antibodies against human Parvovirus B19

Product is intended for research use.
Target Human Parvovirus B19 IgM
Species Reactivity Parvovirus B19
Assay Type Indirect Sandwich ELISA
Applications Lateral Strip Assay
Properties
Background Human Parvovirus B19 occurs endemically world-wide. At intervals of several years, it causes epidemics in small areas from late fall to early summer, affecting kindergartens and schools in particular.Its surface is formed by a protein envelope which consists of two polypeptides (VP-1 and VP-2). VP-1 differs from VP-2 only by an additional N-terminal fragment. Furthermore, a non-structural protein (NS-1) is synthesized, which is required for virus replication.Clinical spectrum of a Parvovirus B19 infection:Fifth disease (erythema infectiosum): It progresses in 20% of cases without symptoms or with flu-like symptoms. Apart from the exanthema, polyarthralgia and a generalized swelling of the lymph nodes can occur occasionally.Aplastic crisis in patients suffering from hemolytic anemias.Spontaneous abortion/hydrops fetalis after infection during pregnancy.Persistent infection in immunosuppressed persons with chronic anemia and bone marrow depression.By the kind of antigen pattern and the avidity determination of IgG antibodies the recomLine Parvovirus B19 provides an indication of the time of infection. The determination of antibodies against the NS-1 antigen can be helpful in the clarification of persistent Parvovirus B19 infections. With the use of VP-2 particles the presentation of conformational epitopes in addition to the linear epitopes is achieved.
Sample Type Serum, plasma, whole blood
Assay Resolution See Figure
Components
10X Wash Buffer100 mL
TMB Substrate40 mL
Milk Powder5 g
Instuctions for Use1 Each
Evaluation Form1 Each
Test Strips2 Vials x 10 Each
Anti-Human IgM Conjugate500 uL
Specificity Information
Target ID Human Parvovirus B19 IgM
Research Areas Infectious Disease
Application Images
Description Strip layout.
Description Test Principle and Procedure:

1. 1st Incubation. A test strip loaded with Parvovirus B19 antigens is incubated with diluted serum or plasma in a dish for
1 hour.
2. Wash 3 times
3. 2nd Incubation
4. Peroxidase conjugated anti-human antibodies (IgG or IgM specific) are added. Incubate for 45 minutes.
5. Wash 3 times
6. Color reaction
7. 8 minutes after addition of the coloring solution, insoluble colored bands develop at the sites on the test strips occupied by antibodies.
Description Related recombinant Parvovirus B19 antigens
Description Example sample data.

Typical Parvovirus B19 course of infection and two examples of healthy blood donors with past infections. The course of infection covers a period of approx. 21 weeks after appearance of symptoms. IgM reactivity decreased significantly between sample 2 and 3. The decrease of the VP-C IgG reactivity, the high avidity and the appearance of NS-1 IgG antibodies indicate a status after infection (sample 3). High avidity appears earliest 4 weeks after infection. NS-1 antibodies titers appear only six to eight weeks after infection, but not in all patients. In a study samples from blood donors were tested, 66% were IgG positive. Many samples presented this typical antigens pattern in past infections as shown. In Germany the infection rate in adults is about 60% and in children between 15-30%.
Additional Information
Handling
Storage Store at 2-8°C.
References & Data Sheet