Anti-Beta-O-Linked N-Acetylglucosamine Mouse Monoclonal Antibody (56603)

$441.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100 ug WB,ICC/IF,ELISA,IHC Species Independent
SKU: 56603 Categories: , ,
Overview
Product Name Anti-Beta-O-Linked N-Acetylglucosamine Mouse Monoclonal Antibody (56603)
Description Anti-Beta-O-Linked N-Acetylglucosamine Mouse Monoclonal Antibody
Target O-GlcNAc
Species Reactivity Species Independent
Applications WB,ICC/IF,ELISA,IHC
Host Mouse
Clonality Monoclonal
Clone ID 9H6
Isotype IgM
Immunogen Synthetic Beta-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).
Properties
Form Liquid
Concentration 1 mg/mL
Formulation PBS pH 7.4, 50% glycerol, 0.09% Sodium azide
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium azide
Buffer Cryopreservative 50% glycerol
Format Purified
Purification PEG Purified
Specificity Information
Specificity Specific for Beta-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with N-Acetylgalactosamine, Glucose, or Galactose modified proteins.
Target Name O-GlcNAc
Target ID O-GlcNAc
Alternative Names Beta-O-Linked N-Acetylglucosamine, O-Linked N-Acetylglucosamine, O-GlcNAc, Beta-O-Linked N-Acetylglucosamine, N-Acetylglucosamine, GlcNac, N-Acetylglucosamine, 2-(Acetylamino)-2-deoxy-D-glucose, N-acetyl-D-glucosamine, NAG, Beta-D-(Acetylamino)-2-deoxy-glucopyranose, beta-N-Acetylglucosamine, N-Acetylglucosamine-modified protein, Beta-N-Acetylglucosamine
Research Areas Cell Signaling | Post-translational Modifications | Glycation
Application Images
Description Western Blot analysis of GlcNAc-BSA Conjugate showing detection of 67 kDa O-GlcNAc protein using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: GlcNAc-BSA. Lane 4: GalNAc-BSA. Lane 5: Galactose-BSA. Lane 6: Glucose-BSA. Lane 7: Citrulline-BSA. Load: 2.0 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6 . Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC (1:25); optimal dilutions for assays should be determined by the user.
References & Data Sheet