Anti-IKKγ (NT) Antibody (2335)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF Human, Mouse, Rat
SKU: 2335 Categories: , ,
Overview
Product Name Anti-IKKγ (NT) Antibody (2335)
Description Anti-IKK γ (NT) Rabbit Polyclonal Antibody
Target IKKγ (NT)
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,ICC,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 400-416 of human IKKg. This sequence is identical to that of mouse.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat IKKg (52 kD). No cross-reactivity with IKKalpha, IKKb or IKKepsilon.
Target Name NF-κ-B essential modulator
Target ID IKKγ (NT)
Uniprot ID Q9Y6K9
Alternative Names NEMO, FIP-3, IkB kinase-associated protein 1, IKKAP1, Inhibitor of nuclear factor κ-B kinase subunit γ, I-κ-B kinase subunit γ, IKK-γ, IKKG, IkB kinase subunit γ, NF-κ-B essential modifier
Gene Name IKBKG
Gene ID 8517
Accession Number NP_001093326
Sequence Location Cytoplasm, Nucleus
Biological Function Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor (PubMed:9751060, PubMed:14695475, PubMed:20724660). Its binding to scaffolding polyubiquitin plays a key role in IKK activation by multiple signaling receptor pathways (PubMed:16547522, PubMed:18287044, PubMed:19033441, PubMed:21606507, PubMed:27777308, PubMed:19185524, PubMed:33567255). Can rPubMed:14695475, PubMed:16547522, PubMed:18287044, PubMed:19033441, PubMed:19185524, PubMed:19854139, PubMed:20724660, PubMed:21606507, PubMed:27777308, PubMed:33567255, PubMed:9751060}.; (Microbial infection) Also considered to be a mediator for HTLV-1 Tax oncoprotein activation of NF-kappa-B. {PubMed:10364167, PubMed:11064457}.
Research Areas Neuroscience
Background Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and key mediator of gene expression during immune and inflammatory responses. NF-kappaB activates numerous genes in response to extracellular stimuli, such as IL-1, TNFalpha, and LPS. NF-kappaB is associated with IkappaB in cytoplasm, which inhibits NF-kappaB activity. IkappaB is phos-phorylated by the IkappaB kinase (IKK) complex which contains IKKalpha, IKKbeta, and IKKepsilon. A novel molecule in the IKK complex, designated IKKg, interacts with IKKbeta and is required for the activation of IKK complex and NF-kappaB by LPS, PMA, TNF, and IL-1. IKKg also binds to RIP and NIK and mediates TNF-induced NF-kappaB activation.
Application Images
Description Western blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 ug/mL.
Description Immunocytochemistry of IKK gamma in HeLa cells with IKK gamma antibody at 5 ug/mL.
Description Immunofluorescence of IKK gamma in Hela cells with IKK gamma antibody at 20 ug/mL. Red: IKK gamma Antibody (2335)
Blue: DAPI staining
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500-1:1,000 dilution.

Positive control: Whole cell lysate from HeLa cells.
References & Data Sheet