Anti-Glutathione Reductase Antibody (13901)

$410.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 200ug WB,ELISA Human
SKU: 13901 Categories: , ,
Overview
Product Name Anti-Glutathione Reductase Antibody (13901)
Description Anti-Glutathione Reductase Mouse Monoclonal Antibody
Target Glutathione Reductase
Species Reactivity Human
Applications WB,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 2A2-1B7
Isotype IgG1
Immunogen Recombinant human glutathione reductase purified from E. coli expressing the human glutathione reductase gene (aa 44-522).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification PEG Purified
Specificity Information
Specificity These antibodies recognize human glutathione reductase. They do not cross-react with yeast glutathione reductase.
Target Name Glutathione reductase, mitochondrial
Target ID Glutathione Reductase
Uniprot ID P00390
Alternative Names GR, GRase, EC 1.8.1.7
Gene Name GSR
Accession Number NP_000628
Sequence Location [Isoform Mitochondrial]: Mitochondrion.; [Isoform Cytoplasmic]: Cytoplasm.
Biological Function Maintains high levels of reduced glutathione in the cytosol.
Research Areas Enzymes
Background Glutathione reductase, also known as glutathione-disulfide reductase, is an enzyme that in humans is encoded by the GSR gene. Glutathione reductase catalyzes the reduction of glutathione disulfide to the sulfhydryl form glutathione (GSH), which is a critical molecule in resisting oxidative stress and maintaining the reducing environment of the cell. The importance of the glutathione pathway and enzymes that affect it is gaining an increased level of attention. Although glutathione reductase has been an attractive target for many pharmaceuticals, there have been no successful glutathione reductase related therapeutic compounds created to date.
Application Images
Description ELISA: Titration of glutathione reductase MAbs with glutathione reductase at 3ug/ml on the wells.
Description ELISA Pairs: Both of these antibodies function as capture and detection antibodies at 1ug/ml.
Additional Information
Handling
Storage These antibodies are stable for at least one (1) year at -20°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions ELISA: Titration of glutathione reductase MAbs with glutathione reductase at 3ug/ml on the wells.ELISA Pairs: Both of these antibodies function as capture and detection antibodies at 1ug/ml.

Immunoblotting: use at 0.5-1.0ug/ml. A band of ~57kDa is detected.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet