Anti-GFRα-3 Antibody (1137)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Human, Mouse, Rat
SKU: 1137 Categories: , ,
Overview
Product Name Anti-GFRα-3 Antibody (1137)
Description Anti-GFR-3 (IN) Rabbit Polyclonal Antibody
Target GFRα-3
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 347- 360 of mouse GFRa-3.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat GFRalpha-3 (approx. 43 kD).
Target Name GDNF family receptorα-3
Target ID GFRα-3
Uniprot ID O35118
Alternative Names GDNF receptorα-3, GDNFR-α-3, GFR-α-3
Gene Name Gfra3
Gene ID 14587
Accession Number NP_034410
Sequence Location Cell membrane, Lipid-anchor, GPI-anchor
Biological Function Receptor for the glial cell line-derived neurotrophic factor, artemin. Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase (By similarity). {ECO:0000250}.
Research Areas Neuroscience
Background Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN), play key roles in the control of vertebrate neuronal survivial and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors requrie two receptor subunits, the novel glycosylphosphatidylinositol linked protein GFRalpha and Ret receptor tyrosine kinase GFRbeta. Following the identification of GFRalpha-1 and –2, another receptor in the GFR family was identified in human and mouse and designated GFRalpha-3. GFRalpha-3 binds persephin. Thus, persephin, GFRalpha-3, and Ret PTK form a complex to transmit the persephin signal and to mediate persephin function.
Application Images
Description Western blot analysis of GFR alpha 3 in crude cell membrane fractions of mouse heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with GFR alpha 3 antibody at 1 2 ug/mL.
Description Immunohistochemistry of GFR alpha 3 in mouse heart tissue with GFR alpha 3 antibody at 10 ug/mL.
Description Immunofluorescence of GFR alpha 3 in mouse kidney tissue with GFR alpha 3 antibody at 5 ug/ml. Green: GFR alpha 3 antibody (1137)
Red: Phylloidin staining
Blue: DAPI staining
Description Immunohistochemistry of GFR alpha 3 in mouse kidney tissue with GFR alpha 3 antibody at 5 ug/ml.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500-1:1,000 dilution.

Positive control: Tissue lysates of mouse kidney, liver, or heart.
References & Data Sheet