Anti-ENaCα Antibody (11574)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC Mouse, Rat
SKU: 11574 Categories: , ,
Overview
Product Name Anti-ENaCα Antibody (11574)
Description Anti-ENaCα Mouse Monoclonal Antibody
Target ENaCα
Species Reactivity Mouse, Rat
Applications WB,IHC
Host Mouse
Clonality Monoclonal
Clone ID 1.40E+11
Isotype IgG1
Immunogen Synthetic peptide corresponding to N-terminal aa 46-68 of rat ENaC alpha.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Concentration: 1mg/ml
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity Mouse and rat ENaC alpha
Target Name Amiloride sensitive sodium channelα1 subunit
Target ID ENaCα
Uniprot ID Q6IRJ1
Gene Name Scnn1a
Accession Number NP_113736
Research Areas Ion Channels
Background The epithelial sodium channel (ENaC) is a membrane-bound ion-channel that is selectively permeable to Na+ ions and that is assembled as a heterotrimer composed of three homologous subunits alpha, beta, and g. It is involved primarily in the reabsorption of sodium ions in the collecting ducts of the kidney's nephrons. These channels mediate the first step of active sodium reabsorption essential for the maintenance of body salt and water homeostasis. In vertebrates, the channels control reabsorption of sodium in kidney, colon, lung and sweat glands; they also play a role in taste perception.
Application Images
Description Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 14E10 (11574). Tissue: Kidney. Species: Rat. Fixation: Paraffin-embedded formalin-fixed. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody (11574) at 1:100. Secondary Antibody: Goat Anti-Mouse ATTO 488 (green). Localization: Intercalated cells. Aquaporin 2 Antibody staining in red.
Description Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~85kDa ENaC alpha protein using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 14E10 (11574). Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 µg. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody (11574) at 1:1000. Predicted/Observed Size: ~85kDa.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Immunofluorescence:Detection of ~85kDa ENaC alpha with #11574 at 1ug/mL.Detection of ENaC alpha (green) in paraffin- embedded, formalin-fixed rat kidney with #11574 at 10ug/mL.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet