Anti-Elastase (Neutrophil) Mouse Monoclonal Antibody – Library Pack (132101)

$531.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 4 x 100ug ELISA Human
SKU: 132101 Categories: , , ,
Overview
Product Name Anti-Elastase (Neutrophil) Mouse Monoclonal Antibody - Library Pack (132101)
Description Anti-Elastase (Neutrophil) Mouse Monoclonal Antibody Library Pack - Includes 100 ug of each of the following antibodies:

13201 - Anti-Elastase (Neutrophil) Mouse Monoclonal Antibody - Clone ELA11-207.2 - Isotype IgM

13202 - Anti-Elastase (Neutrophil) Mouse Monoclonal Antibody - Clone ELA11-207.3 - Isotype IgM

13203 - Anti-Elastase (Neutrophil) Mouse Monoclonal Antibody - Clone ELA10-101.5 - Isotype IgG1

13204 - Anti-Elastase (Neutrophil) Mouse Monoclonal Antibody - Clone ELA10-103.2 - Isotype IgG2b

Target Elastase (Neutrophil)
Species Reactivity Human
Applications ELISA
Host Mouse
Clonality Monoclonal
Immunogen Purified human neutrophil elastase.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation PBS
Buffer pH 7.4
Format Purified
Specificity Information
Specificity These antibodies recognize human neutrophil elastase and vary in their ability to inhibit neutrophil elastase activity as determined in an assay of elastase-mediated hydrolysis of elastin-Congo Red substrate:
Target Name Neutrophil elastase
Target ID Elastase (Neutrophil)
Uniprot ID P08246
Gene Name ELANE
Gene ID 130130
Sequence Location Cytoplasmic vesicle, phagosome
Biological Function Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984). {PubMed:10947984, PubMed:15140022}.
Research Areas Enzymes
Background Neutrophil elastase is a major neutral protease present in human neutrophils and is released by neutrophils following stimulation with a variety of stimuli. It is believed to be a major mediator of the lung destruction seen in emphysema. In addition to elastin, neutrophil elastase can cleave other biologically active proteins such as fibrinogen and antithrombin and is released by neutrophils during clot formation.
Application Images
Description These antibodies recognize human neutrophil elastase and vary in their ability to inhibit neutrophil elastase activity as determined in an assay of elastase-mediated hydrolysis of elastin-Congo Red substrate:
Additional Information
Handling
Storage These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions These antibodies have been qualified for use in ELISA to detect human neutrophil elastase and in studies of inhibition and enzyme kinetics of human neutrophil elastase.
References & Data Sheet