Mikrogen Diagnostik recomLine Cytomegalovirus IgM Lateral Strip Test Kit (5573)

$660.00

Host Quantity Applications Species Reactivity Data Sheet  
20 Tests Lateral Strip Assay Cytomegalovirus
SKU: 5573 Categories: ,
Overview
Product Name Mikrogen Diagnostik recomLine Cytomegalovirus IgM Lateral Strip Test Kit (5573)
Description Line immunoassay using recombinant antigens to detect IgG and IgM antibodies directed against Cytomegalovirus (Cytomegalovirus) and to determine Cytomegalovirus-IgG avidity Cytomegalovirus (Cytomegalovirus)

Product is intended for research use.
Target Human Cytomegalovirus IgM
Species Reactivity Cytomegalovirus
Assay Type Indirect Sandwich ELISA
Applications Lateral Strip Assay
Properties
Background Infections with human cytomegalovirus (Cytomegalovirus, HHV5) normally cause mild symptoms, many patients even remain asymptomatically. Immunocompromised patients and pregnant women however are at high risk for suffering damage from a Cytomegalovirus infection. Under immunosuppressive conditions Cytomegalovirus infection or reactivation can cause a severe clinical course of disease. Pregnant women acquiring a primary infection in approximately 40 percent of the cases transmit the virus to the fetus. About 10 percent of the infected newborns show severe damage at birth or are going to develop long-term effects. Cytomegalovirus infection in early pregnancy may lead to abortion or severe clinical symptoms of the affected children, whereas infection in late pregnancy mostly results in mild cases of illness, or even in asymptomatic newborns.The recomLine Cytomegalovirus is a line immunoassay using all diagnostic relevant Cytomegalovirus antigens in a recombinant format optimized to highest performance in sensitivity and specificity at the same time. Purpose of the assay is the confirmation of positive and unclear screening results (confirmatory assay). Especially the low predictive value of positive IgM results due to persistent or recurrent infections has to be clarified. The test system either is able to determine IgG and IgM antibodies and the avidity of IgG antibodies. Using phase-specific antigens and separate avidity antigens (patented method from MIKROGEN on line immunoassays) the test system can detect Cytomegalovirus specific antibodies in all phases of the infection. Despite viral load the recomLine Cytomegalovirus can be used in pregnancy diagnostic or pre-surgical diagnostic of transplant settings to precisely state if the patient can be judged acute infected or shows typical antigen patterns of a long past infection. While supporting a clear answer to the question what impact the detected Cytomegalovirus antibodies for this particular patient have, this assay can help to optimize therapy, improve disease burden and give patient and doctor relief on reduced acuteness.
Sample Type Serum, plasma, whole blood
Assay Resolution See Figure
Components
10X Wash Buffer100 mL
TMB Substrate40 mL
Milk Powder5 g
Instuctions for Use1 Each
Evaluation Form1 Each
Test Strips2 Vials x 10 Each
Anti-Human IgM Conjugate500 uL
Specificity Information
Target ID Human Cytomegalovirus IgM
Research Areas Infectious Disease
Application Images
Description Strip layout.
Description Recombinant CMV Antigens.
Description Test Principle and Procedure.

1. 1st Incubation. A test strip loaded with CMV antigens is incubated with diluted serum or plasma in a dish for 1 hour.
2. Wash 3 times
3. 2nd Incubation
4. Peroxidase conjugated anti-human antibodies (IgG or IgM specific) are added. Incubate for 45 minutes.
5. wash 3 times
6. Color reaction. 8 minutes after addition of the coloring solution, insoluble colored bands develop at the sites on the test strips occupied by antibodies.
Description Test evaluation, sensitivity and specificity.
Additional Information
Handling
Storage Store at 2-8°C.
References & Data Sheet