Anti-BDNF Antibody (56572)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ICC/IF Human, Mouse
SKU: 56572 Categories: , ,
Overview
Product Name Anti-BDNF Antibody (56572)
Description Anti-BDNF Rabbit Polyclonal Antibody
Target BDNF
Species Reactivity Human, Mouse
Applications WB,ICC/IF
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to the N-terminus of human BDNF.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human and mouse BDNF.
Target Name Brain-derived neurotrophic factor
Target ID BDNF
Uniprot ID P23560
Alternative Names BDNF, Abrineurin [Cleaved into: BDNF precursor form
Gene Name BDNF
Sequence Location Secreted
Biological Function Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. {PubMed:11152678, PubMed:12553913, PubMed:29909994}.; [BDNF precursor form]: Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2 (PubMed:24908487, PubMed:29909994). Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse (By similarity). {UniProtKB:P21237, PubMed:24908487, PubMed:29909994}.
Research Areas Neuroscience
Background BDNF acts on neurons of the central nervous system and the peripheral nervous system supporting the survival of existing neurons and stimulating growth and differentiation of new neurons and synapses. In the brain, it is active in the hippocampus, cortex, and basal forebrain, areas vital to learning, memory, and higher thinking. It is also expressed in the retina, motor neurons, kidneys, saliva, and prostate.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BDNF Polyclonal Antibody (56572). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (56572) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Secreted, Cytoplasm, Membrane-bound vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BDNF Antibody (D) Composite.
Description Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody (56572). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (56572) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda.
Description Western blot analysis of Mouse Brain showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody (56572). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (56572) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:1,000. A band of ~27-28kDa is detected.

Immunofluorescence: use at dilution of 1:100

These are recommended working dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet