Anti-Arginase-1 Antibody (34051)

$229.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ul IHC Human
SKU: 34051 Categories: , , Tag:
Overview
Product Name Anti-Arginase-1 Antibody (34051)
Description Anti-Arginase-1 Mouse Monoclonal Antibody
Target Arginase-1
Species Reactivity Human
Applications IHC
Host Mouse
Clonality Monoclonal
Clone ID G400.1
Isotype IgG1
Immunogen Recombinant human Arginase-1
Properties
Form Liquid
Concentration Lot Specific
Formulation Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.
Buffer Formulation Tris
Buffer pH pH 7.3-7.7
Buffer Anti-Microbial 0.1% Sodium Azide
Buffer Protein Stabilizer 1% Bovine Serum Albumin
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity Human Arginase-1. Reactivity with other species has not been investigated.
Target Name Arginase-1
Target ID Arginase-1
Uniprot ID P05089
Alternative Names EC 3.5.3.1, Liver-type arginase, Type I arginase
Gene Name ARG1
Sequence Location Cytoplasm, Cytoplasmic granule
Biological Function Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. {UniProtKB:Q61176, PubMed:15546957, PubMed:16709924, PubMed:19380772}.
Research Areas Cancer research
Background The product of the ARG1 gene, Arginase-1, is a cytosolic metalloenzyme expressed predominantly in hepatocytes and plays an important role in the urea cycle by catalyzing the hydrolysis of arginine to ornithine and urea. Argininemia is an inherited autosomal recessive disorder characterized by a buildup of arginine and ammonia in the bloodstream. Arginase-1 has been identified as a specific marker of benign and malignant hepatic tumors.
Application Images
Description Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes. Detection of Arginase-1 in human liver with #34051 diluted 1:100-1:200.
Additional Information
Handling
Storage Store at 2-8°C. Do not freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions

Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.
References & Data Sheet