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Anti-α-1-antitrypsin Mouse Monoclonal Antibody – Library Pack (137101)

$531.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 4 x 100ug ELISA Human
SKU: 137101 Categories: , , ,
Overview
Product Name Anti-α-1-antitrypsin Mouse Monoclonal Antibody - Library Pack (137101)
Description Anti-α-1-antitrypsin Mouse Monoclonal Antibody Library Pack - Includes 100 ug of each of the following antibodies:

13701 - Anti-α-1-antitrypsin Mouse Monoclonal Antibody - Clone 1AT10-105.10 - Isotype IgG2a

13702 - Anti-α-1-antitrypsin Mouse Monoclonal Antibody - Clone 1AT11-210.1 - Isotype IgG1

13703 - Anti-α-1-antitrypsin Mouse Monoclonal Antibody - Clone 1AT11-209.3 - Isotype IgG1

13704 - Anti-α-1-antitrypsin Mouse Monoclonal Antibody - Clone 1AT11-213.5 - Isotype IgG1

Target α-1-antitrypsin
Species Reactivity Human
Applications ELISA
Host Mouse
Clonality Monoclonal
Immunogen Purified human serum alpha-1-antitrypsin.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity These antibodies specifically recognize human serum alpha-1-antitrypsin. They do not cross-react with alpha-1-antichymotrypsin.
Target Name α-1-antitrypsin
Target ID α-1-antitrypsin
Uniprot ID P01009
Gene Name SERPINA1
Gene ID 107400
Sequence Location Secreted. Endoplasmic reticulum. Note=The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum.; [Short peptide from AAT]: Secreted, extracellular space, extracellular matrix.
Biological Function Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.; [Short peptide from AAT]: Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Research Areas Enzymes
Background Alpha-1 Antitrypsin (alpha1AT) is a protease inhibitor of the serpin superfamily. It is generally known as serum trypsin inhibitor and is also referred to as alpha-1 proteinase inhibitor (alpha1PI) because it inhibits multiple proteases. It protects tissues from proteases of inflammatory cells, particularly neutrophil elastase, and has a reference range in blood of 1.5 – 3.5 gram/liter, but the concentration can rise significantly in cases of acute inflammation. In its absence (such as in alpha1AT deficiency), neutrophil elastase is free to break down elastin, which contributes to the elasticity of the lungs, resulting in respiratory complications such as emphysema, or COPD (chronic obstructive pulmonary disease) in adults and cirrhosis in adults or children.
Application Images
Additional Information
Handling
Storage These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions These antibodies have been qualified for use in ELISA to detect human a1AT.

End users should determine optimal concentrations for their applications.
References & Data Sheet