Specificity Any Detects 90kDa Hsp90b in all reactive species except chicken where it detects both alpha and beta isoforms. This antibody detects 72 and 73kDa proteins corresponding to the predicted molecular masses of Hsp70 and Hsc70, respectively, on immunoblots of human, mouse, rat, rabbit, monkey, hamster, guinea pig, This antibody is reactive with both the constitutive and the inducible forms of human, mouse, rat, rabbit, chicken, Sf9 cell, Achlya, and wheat germ Hsp90. However, it does not bind to the native for This antibody recognizes a ~70 kDa protein corresponding to the predicted molecular mass of HDEL in Drosophila, yeast, and various plants (barnyard grass, beet, cotton, mung bean, sorghum, and wheat) This antibody recognizes an ~85 kDa protein (inactive form) in unstressed cell lysates and an ~95 kDa protein (active form) in heat-shocked cell lysates of human, mouse, rat, rabbit, bovine, guinea p This antibody recognizes an ~98 kDa protein corresponding to the predicted molecular mass of Grp94 on SDS-PAGE immunoblots in human, mouse, rat, rabbit, monkey, hamster, guinea pig, dog, bovine, shee This antibody recognizes constitutive and inducible plant Hsp70. It does not cross-react with human, rat, bacteria (DNAKK) or human BIP. This antibody recognizes eukaryotic Hsp90alphab. This antibody recognizes HSF2 in heat- shocked cell lysates of human, mouse, rat, rabbit, bovine, canine, porcine, guinea pig, hamster, sheep, and monkey. This antibody recognizes Hsp70 of human, mouse, rat, rabbit, monkey, guinea pig, hamster, chicken, bovine, canine, ovine, porcine, fish, Drosophila, and C. elegans and does not cross-react with Hsc70 This antibody recognizes human and bovine alpha B crystallin. It does not cross-react with alpha A crystallin, beta- L crystallin, beta-H crystallin, gamma crystallin, Hsp25, Hsp27, or Hsp47 proteins This antibody recognizes human and mouse Aha1 and cross-reacts with mouse Aha2. This antibody recognizes human and mouse tsp23. Other species have not been investigated. It does not cross-react with p23. This antibody recognizes human and rat TRAP1. Other species have not been investigated. This antibody recognizes human Grp170 (170kDa). This antibody recognizes human GRP78. This antibody recognizes human Hsp27. This antibody recognizes human Hsp47. This antibody recognizes human TRAP1. Other species have not been investigated. This antibody recognizes human, mouse, and rat Grp170 (170kDa). This antibody recognizes human, mouse, and rat GRP78. This antibody recognizes human, mouse, and rat Hsc70. Other species have not been tested. It does not cross-react with Hsp70. This antibody recognizes human, mouse, and rat Hsp22. It detects endogenous and exogenous Hsp22 in monomeric, dimeric, and tetrameric (weak) forms in Western blots. Exogenous Hsp22 is detected in dim This antibody recognizes human, mouse, and rat Hsp40 (Hdj1). Other species have not been tested. It does not cross-react with Hdj2 or Ydj1. This antibody recognizes human, mouse, and rat Hsp90alpha and b. Other species have not been tested. This antibody recognizes human, mouse, and rat Hsp90alpha. Other species have not been tested. It does not cross-react with Hsp90b in ELISA. This antibody recognizes human, mouse, fish, rat, avian, amphibian, Drosophila, and yeast Hsp70, Hsc70, and, following heat shock, Hsp72. This antibody recognizes human, mouse, rat, and bovine alpha A crystallin. Other species have not been tested. It does not cross-react with alpha B crystallin, beta-L crystallin, beta- H crystallin, This antibody recognizes human, mouse, rat, beluga, bovine, canine, fish, guinea pig, hamster, monkey, porcine, ovine, and coral Hsp70. This antibody recognizes human, mouse, rat, bovine, and chicken alpha B crystallin. It does not cross-react with alpha A crystallin. This antibody recognizes human, mouse, rat, bovine, canine, guinea pig, and hamster Hsp 25/27. Some cross- reactivity with alpha B crystallin. This antibody recognizes human, mouse, rat, dog, and hamster FKBP52. This antibody recognizes human, mouse, rat, fish, avian, amphibian, Drosophila, and yeast Hsp70, Hsc70, p75, and, following heat shock, Hsp72. This antibody recognizes human, mouse, rat, fish, avian, rabbit, amphibian, Drosophila, and yeast Hsp70, Hsc70, Grp78, and, following heat shock, Hsp72. This antibody recognizes human, mouse, rat, rabbit, bovine, canine, porcine, guinea pig, hamster, chicken, monkey, white fly, yeast and bacterial* Hsp60 (60 kDa). The epitope recognized is within aa This antibody recognizes human, mouse, rat, rabbit, bovine, canine, porcine, ovine, guinea pig, hamster, chicken, monkey, Xenopus, and Drosophila Hsp60 (60 kDa). It does not cross-react with bacteria This antibody recognizes human, mouse, rat, rabbit, bovine, porcine, and chicken Hsp90alpha (90 kDa). This antibody recognizes human, rat, and mouse Hsp90b. It does not cross- react with Hsp90alpha. This antibody recognizes inducible and constitutive Hsp70/Hsc70 of human, mouse, rat, rabbit, monkey, guinea pig, hamster, chicken, bovine, canine, ovine, porcine, fish, Xenopus, Drosophila, C. elega This antibody recognizes inducible bovine, human, mouse and rat Hsp70. It does not cross-react with Hsc70. This antibody recognizes P. falciparum Hsp60 and cross-reacts with E.coli Hsp60/GroEl. This antibody recognizes P. falciparum Hsp70. This antibody recognizes yeast Hsp40 (Ydj1). It does not cross-react with human, mouse, or rat Hsp40. Binds multiple linear epitopes within human alpha synuclein: 21-70 aa, 61-90 aa, and 101-140 aa, determined using Dot blot and ELISA with corresponding peptides. Detects ~14 kDa band corresponding to Binds within the 101-130 aa region of human alpha synuclein, determined using Dot blot and ELISA with corresponding peptides. Detects ~14 kDa band corresponding to Alpha synuclein. Band at ~40 kDa is Binds within the 101-140 aa region of human alpha synuclein, determined using WB and ELISA with corresponding peptides. Detects ~14 kDa band corresponding to Alpha synuclein. Does not detect truncat Binds within the 81-130 aa region of human alpha synuclein, determined using WB and ELISA with corresponding peptides. Detects ~14 kDa band corresponding to Alpha synuclein. Band at ~30 kDa is a dime Detects ~140kDa. Weak human detection. Does not cross-react with KCNT2/Slo2.1/Slick. Detects 26 kDa for purified GST protein. Does not detect proteins without a GST tag. Detects 36 kDa band corresponding to Pseudomonas aeruginosa Metallothionein. Specific for 3,5-Dibromo-tyrosine. Does not cross-react with Nitrotyrosine. Specific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxy-2-hexenal (4-HHE) or 4-Hydroxynonenal (4-HNE). Does not cross-react with Acrole Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehy Specific for 6-Nitrotyrptophan modified proteins. Doss not cross-react with 7-Ketocholoesterol modified proteins. Specific for 7-ketocholesterol modified proteins. Specific for Acrolein modified proteins. Does not detect free acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylgl Specific for Beta-O-Linked N-Acetylgalactosamine and Beta-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with Glucose or Galactose modified proteins. Specific for Beta-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with N-Acetylgalactosamine, Glucose, or Galactose modified proteins. Specific for citrulline residues on modified proteins. Tested using citrulline conjugated Bovine Serum Albumin (BSA). Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins. Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy none Specific for Malondialdehyde conjugated proteins. Does not detect free Malondialdehyde. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crot Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.
