Specificity Any Antibody is affinity-purified on human IgE and conjugated to fluorescein isothiocyanate (FITC). Fluorophore:antibody ratio = 4.7. Cross-reactivity with other immunoglobulins is negligible. Antibody is affinity-purified on human IgG. By immunoblotting and ELISA this antibody reacts specifically with human IgG and light chains common to other human immunoglobulins. This antibody may cros Antibody is affinity-purified on human IgM and conjugated to alkaline phosphatase. This antibody may cross-react with IgM of other species. Antibody is affinity-purified on human IgM and conjugated to fluorescein isothiocyanate (FITC). Fluorophore:protein ratio = 5.8. This antibody may cross-react with IgM of other species. Antibody is affinity-purified on mouse IgA. Cross-reactivity with mouse IgM, IgG1, IgG2a, IgG2b, IgG2c, IgG3, and IgE is not detectable. Antibody is affinity-purified on mouse IgE. Cross-reactivity with other immunoglobulins is negligible. Antibody is affinity-purified on mouse IgG1. Cross-reactivity with mouse IgA, IgM, IgG2a, IgG2b, IgG2c, IgG3, and IgE is not detectable. Antibody is affinity-purified on mouse IgG2a. Cross-reactivity with other mouse immunoglobulins is <2%. This antibody may cross-react with IgG2a of other species. Antibody is affinity-purified on mouse IgG2b. Cross-reactivity with mouse IgA, IgM, IgG1, IgG2a, IgG2c, IgG3, and IgE is not detectable. Antibody is affinity-purified on mouse IgG2c. By immunoblotting and ELISA, this antibody reacts specifically with IgG2c of C57Bl/6, SJL, C57BL/10, CB20, and C57BL/6 x Balb/c mice. Cross-reactivity wi Antibody is affinity-purified on mouse IgG3. By immunoblotting and ELISA this antibody reacts specifically with mouse IgG3. Cross-reactivity with mouse IgM, IgA, IgG1, IgG2a, IgG2b, IgG2c, and IgE is Antibody is affinity-purified on mouse IgM. By immunoblotting and ELISA this antibody reacts specifically with mouse IgM. Cross-reactivity with mouse IgG subclasses and IgA is negligible. This antibo Antibody is affinity-purified on rat IgA and conjugated to alkaline phosphatase. Molar enzyme:antibody ratio = 1:1. This antibody may cross-react with IgA of other species. Antibody is affinity-purified on rat IgA and conjugated to biotin. Biotin:antibody ratio = 4:1. This antibody may cross-react with IgA of other species. Antibody is affinity-purified on rat IgA and conjugated to fluorescein isothiocyanate (FITC). Fluorophore:antibody ratio = 5.3. This antibody may cross-react with IgA of other species. Antibody is affinity-purified on rat IgA and conjugated to horseradish peroxidase (HRP). Molar enzyme:antibody ratio = 4:1. This antibody may cross-react with IgA of other species. Antibody is affinity-purified on rat IgG and conjugated fluorescein isothiocyanate (FITC). Fluorophore:antibody ratio = 5. This antibody may cross-react with IgG of other species. Antibody is affinity-purified on rat IgG and conjugated horseradish peroxidase (HRP). Molar enzyme:antibody ratio = 4:1. This antibody may cross-react with IgG of other species. Antibody is affinity-purified on rat IgG and conjugated to alkaline phosphatase. Molar enzyme:antibody ratio = 1:1. This antibody may cross-react with IgG of other species. Antibody is affinity-purified on rat IgG and conjugated to biotin. Biotin:antibody ratio = 4:1. This antibody may cross-react with IgG of other species. Antibody is affinity-purified on rat IgG1 and conjugated to alkaline phosphatase. Molar enzyme:antibody ratio = 1:1. This antibody may cross-react with IgG1 of other species. Antibody is affinity-purified on rat IgG1 and conjugated to fluorescein isothiocyanate. Fluorophore:antibody ratio = 5.1. This antibody may cross-react with IgG1 of other species. Antibody is affinity-purified on rat IgG1 and conjugated to horseradish peroxidase (HRP). Molar enzyme:antibody ratio = 4:1. This antibody may cross-react with IgG1 of other species. Antibody is affinity-purified on rat IgG2a and conjugated to alkaline phosphatase. Molar enzyme:antibody ratio = 1:1. This antibody may cross-react with IgG2a of other species. Antibody is affinity-purified on rat IgG2a and conjugated to fluorescein isothiocyanate (FITC). Fluorophore:antibody ratio = 5.6. This antibody may cross-react with IgG2a of other species. Antibody is affinity-purified on rat IgG2a and conjugated to horseradish peroxidase (HRP). Molar enzyme:antibody ratio = 4:1. This antibody may cross-react with IgG2a of other species. Antibody is affinity-purified on rat IgG2c and conjugated to alkaline phosphatase. Molar enzyme:antibody ratio = 1:1. This antibody may cross-react with IgG2c of other species. Antibody is affinity-purified on rat IgG2c and conjugated to fluorescein isothiocyanate. Fluorophore:antibody ratio = 6.2. This antibody may cross-react with IgG2c of other species. Antibody is affinity-purified on rat IgG2c and conjugated to horseradish peroxidase. Molar enzyme:antibody ratio = 4:1. This antibody may cross-react with IgG2c of other species. Antibody is affinity-purified on rat IgM and conjugated to alkaline phosphatase. Molar enzyme:antibody ratio = 1:1. This antibody may cross-react with IgM of other species. Antibody is affinity-purified on rat IgM and conjugated to biotin. Biotin:antibody ratio = 4:1. This antibody may cross-react with IgM of other species. Antibody is affinity-purified on rat IgM and conjugated to fluorescein isothiocyanate (FITC). Fluorophore:antibody ratio = 7.7. This antibody may cross-react with IgM of other species. Antibody is affinity-purified on rat IgM and conjugated to horseradish peroxidase (HRP). Molar enzyme:antibody ratio = 4:1. This antibody may cross-react with IgM of other species. Antibody was affinity-purified on GST. Antibody was affinity-purified on the peptide. Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins. Antigen: Synthetic peptide corresponding to aa 410-419 (EQKLISEEDL) of human myc conjugated to KLH. Antiserum was cross-adsorbed on bovine, chicken, horse, mouse, pig, rabbit, and rat immunosorbents to remove cross-reactive antibodies. By immunoelectrophoresis and ELISA this antibody reacts specifi By immunoblotting and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. This antibody may cross-react with IgG of other species. By immunoblotting and ELISA this antibody reacts specifically with mouse IgG. Cross-reactivity with mouse IgA and IgM is negligible. This antibody may cross-react with IgG of other species. By immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgY and with light chains common to other chicken immunoglobulins. Reactivity with non- immunoglobulin serum proteins By immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgY and with light chains common to other chicken immunoglobulins. Reactivity with other non-immunoglobulin serum pro By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross-reactivity with IgG, IgM and light chains is less than 0.1%. This antibody may cross-react with IgA from oth By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross-reactivity with IgG, IgM and light chains is less than 0.1%. This antibody may cross-react with IgA of other By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgE. By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. Cross-reactivity with non-immunoglobulin serum protein By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgM. Cross-reactivity with other immunoglobulins and light chains is less than 0.1%. This antibody may cross-react with By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. Reactivity with non- immunoglobulin serum proteins is By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG- Fc. Cross-reactivity with IgA and IgM is negligible. This antibody may cross-react with IgG from other species. By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. Reactivity with non- immunoglobulin serum proteins i By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG Fc fragment. This antibody may cross-react with IgG from other species. By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG Fc fragment. This antibody may cross-react with IgG of other species. By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG- Fc. Reactivity with Fab fragments is not detected. This antibody may cross-react with IgG from other species. By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG- Fc. Reactivity with rabbit Fab fragments is not detected. This antibody may cross-react with IgG from other speci By immunoelectrophoresis and ELISA, this antibody reacts specifically with human IgM. Cross-reactivity with other immunoglobulins and light chains is less than 0.1%. This antibody may cross-react wit
