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Anti-8-oxo-Adenosine DNA/RNA Damage Monoclonal Antibody (12503)

$230.00 – $460.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug
500ug 		ELISA Human
SKU: 12503 Categories: , ,
Overview
Product Name 8-Oxo-A DNA/RNA Damage Monoclonal Antibody (12503)
Description Anti-8-oxoA, a mouse monoclonal antibody targeting oxidative damage in RNA, DNA, ATP and other nucleotide precursors and degradation products
Target 8-Oxo-Adenosine
Species Reactivity Human
Applications ELISA
Host Mouse
Clonality Monoclonal
Clone ID 6E4
Isotype IgG1κ
Immunogen 8-Oxo-A conjugated to KLH
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Protein G Affinity Chromatography
Specificity Information
Specificity This antibody recognizes 8-oxoA in single and double stranded RNA and DNA and also in free nucleotides such as 8-oxoATP
Target Name 8-Oxo-Adenosine
Target ID 8-Oxo-Adenosine
Alternative Names 8-oxoA, 8-Oxoadenosine, 8-Hydroxyadenosine, 7,8-dihydro-8-oxoadenosine, 6-Azanyl-9-[(2r,3r,4s,5r)-3,4-Dihydroxy-5-(Hydroxymethyl)oxolan-2-Yl]-7h-Purin-8-One
Research Areas DNA/RNA Damage
Application Images
Description Determined by a competitive inhibition ELISA with nucleoside variants. The %B/B0 is plotted as a function of the concentration (nM) of the indicated competitor. 100% B/B0 indicates no inhibition by the indicated competitor.
Description Selectivity and Specificity of 6E4 in RNA:
  • Binds 8-oxoA in single and double stranded RNA
  • Recognizes 8-oxoA in RNA base mispairs with standard geometry
Description Selectivity and Specificity of 6E4 in DNA:
  • Binds 8-oxoA in single and double stranded DNA
  • Recognizes 8-oxoA in all DNA base mispairs
Additional Information
Handling
Storage Store antibody at -20C to -80C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system. Optimal concentrations must be derived for specific applications.
References & Data Sheet
References Daniel J. Tew a, Jakob M. Hebert b, Brad J. Schmier, Discovery and properties of a monoclonal antibody targeting 8-oxoA, an oxidized adenine lesion in DNA and RNA, Redox Biology 62 (2023) 102658, PMC10074937