Anti-Citrulline Mouse Monoclonal Antibody (56601)

$441.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100 ug WB,ICC/IF,ELISA,IHC Species Independent
SKU: 56601 Categories: , ,
Overview
Product Name Anti-Citrulline Mouse Monoclonal Antibody (56601)
Description Anti-Citrulline Mouse Monoclonal Antibody
Target Citrulline
Species Reactivity Species Independent
Applications WB,ICC/IF,ELISA,IHC
Host Mouse
Clonality Monoclonal
Clone ID 2D3.1
Isotype IgG1
Immunogen Synthetic L-Citrulline conjugated to Keyhole Limpet Hemocyanin (KLH).
Properties
Form Liquid
Concentration 1 mg/mL
Formulation PBS pH 7.4, 50% glycerol, 0.09% Sodium azide
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium azide
Buffer Cryopreservative 50% glycerol
Format Purified
Purification Protein G Purified
Specificity Information
Specificity Specific for citrulline residues on modified proteins. Tested using citrulline conjugated Bovine Serum Albumin (BSA).
Target Name Citrulline
Target ID Citrulline
Alternative Names L-Citrulline, 2-Amino-5-(carbamoylamino)pentanoic acid, Citrulline, Citrulline, Citrulline-modified protein, ACPA, Anti-citrullinated protein antibodies, Anti-citrullinated protein, Modified Citrulline, Citrulline-containing protein, Citrullinated protein
CAS Number 627-77-0
PubChem ID 833
Research Areas Cell Signaling | Post-translational Modifications | Citrullination
Application Images
Description Western Blot analysis of Citrulline-BSA Conjugate showing detection of 67 kDa Citrulline protein using Mouse Anti-Citrulline Monoclonal Antibody, Clone 2D3-1B9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (2.0 µg). Lane 4: Citrulline-BSA (0.5 µg). Lane 5: Citrulline-BSA (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 2D3.1 . Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 2D3.1 . Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC (1:100); optimal dilutions for assays should be determined by the user.
References & Data Sheet