BriClone SF Hybridoma Cloning Medium (BR-002)

$450.00

Note: This product has a $35.00 Dry Ice Fee.
Host Quantity Applications Species Reactivity Data Sheet  
100 mL Cell Culture
SKU: BR-002 Category: Tag:
Overview
Product Name BriClone SF Hybridoma Cloning Medium (BR-002)
Description BriClone SF is a Serum Free additive for the cloning medium used in the post-fusion stages of hybridoma production and for improving the efficiency of hybridoma cells cloning.
Applications Cell Culture
Properties
Form Liquid
Concentration 20X Solution
Background BriClone SF completely replaces the need for feeder cells. The use of feeder cells to support the outgrowth of hybridomas has many disadvantages, like batch-to-batch variation of the feeder cells and competition between feeder cells and freshly-fused hybridomas for nutrients in the culture medium. BriClone has been specially developed to overcome these common problems in the post-fusion period.
Specificity Information
Application Images
Description Percentage of fusion efficiency from freshly fused hybridomas cultured using BriClone (red) compared to Bri-Clone-SF (brown) and a negative control (blue).
Description BriClone-SF has equivalent fusion efficiency activity when compared to two leading competitor products when used at the manufacturer’s recommended concentrations. Percentage of fusion efficiency from freshly fused hybridomas cultured using BriClone-SF (orange) compared to Competitor A (light grey) and competitor B (dark grey) products and a negative control (blue).
Description BriClone-SF is stable for at least 9 months when stored at -20˚C. Percentage of fusion efficiency from freshly fused hybridomas cultured using BriClone-SF stored at 4˚C for 2 weeks, Room Temperature for 1 week and -20˚C for 9 months compared to a negative control (blue).
Additional Information
Additional Information BriClone is produced by exposing basal culture medium with a low percentage of foetal calf serum to a human cancer cell line, which secretes relevant molecules into the medium, resulting in a product consistently upportive of the fusion and cloning stages of hybridoma production. Some researchers require a product which is serum-free and has had no contact with human or animal cells. We have, therefore, designed BriClone-SF which has the same basal medium and biological activities as BriClone but has no added serum or other substances of human or animal origin and has not been exposed to cells. BriClone-SF contains added recombinant human proteins produced in bacterial or yeast host cells.

Quality Control
Each batch of BriClone-SF is tested for its ability to support / promote the growth of newly PEG fused hybridoma cells, plated in 48 well plates containing HAT selection medium with 5% BriClone-SF over a 10-12 day time period. Test cells used are Sp2/0-Ag14 mouse myeloma cells and immune splenocytes isolated from BALB/c mice.

-This product is for in vitro research purposes only. Not for human or veterinary use.
-Always use BriClone under aseptic conditions
-This product has been produced using cells that have not been screened for Hepatitis B, Human immunodeficiency viruses or other agents.
-Handle as a potentially biohazardous material under at least Biosafety Level 1 containment.
Handling
Storage Store at -20˚C. Avoid repeated freeze/thaw cycles. Stable for short periods at +4˚C
Dilution Instructions Thaw and add to the hybridoma cloning medium as a 5% v/v supplement.
Application Instructions Thaw and add to the hybridoma cloning medium as a 5% v/v supplement. BriClone SF can be used for:
• Hybridoma growth post-fusion (refer to your own protocol)
• Hybridoma Cloning (refer to your own protocol)
References & Data Sheet
References PMC7873262,PMC7239657,PMC6803335,PMC6816435,PMC6719973,PMC5017023,PMC2812581,PMC2896538,PMC4617144,PMC3944398,PMC9030397,PMC8470961,PMC8104110,PMC7279471,PMC7096428,PMC5992998,PMC5986985,PMC5857169,PMC4921032,PMC4591809,PMC4135088,PMC1858089,PMC1602225
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Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C44Mab-5 Shinji Yamada, Shunsuke Itai, Takuro Nakamura, Miyuki Yanaka, Mika K. Kaneko, Yukinari Kato Biochem Biophys Rep. 2018 Jul; 14: 64–68. Published online 2018 Apr 12. doi: 10.1016/j.bbrep.2018.03.007

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Cellular Interaction and Cytotoxicity of the Iowa Mutation of Apolipoprotein A-I (ApoA-IIowa) Amyloid Mediated by Sulfate Moieties of Heparan Sulfate Kaori Kuwabara, Kazuchika Nishitsuji, Kenji Uchimura, Shang-Cheng Hung, Makoto Mizuguchi, Hiroyuki Nakajima, Shiho Mikawa, Norihiro Kobayashi, Hiroyuki Saito, Naomi Sakashita J Biol Chem. 2015 Oct 2; 290(40): 24210–24221. Published online 2015 Aug 19. doi: 10.1074/jbc.M115.652545

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