Anti-DNA Polymeraseδ Antibody (13910)

$503.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC-P Human
SKU: 13910 Categories: , ,
Overview
Product Name Anti-DNA Polymeraseδ Antibody (13910)
Description Anti-DNA Polymeraseδ Mouse Monoclonal Antibody
Target DNA Polymeraseδ
Species Reactivity Human
Applications WB,IHC-P
Host Mouse
Clonality Monoclonal
Clone ID 6196
Isotype IgG1
Immunogen Recombinant protein corresponding to aa 1-140 of the large subunit of human DNA polymerase delta
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.2.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human DNA polymerase delta.
Target Name DNA polymeraseδ catalytic subunit
Target ID DNA Polymeraseδ
Uniprot ID P28340
Alternative Names EC 2.7.7.7, 3'-5' exodeoxyribonuclease, EC 3.1.11.-, DNA polymerase subunitδ p125
Gene Name POLD1
Accession Number NP_001243778.1
Sequence Location Nucleus
Biological Function As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24035200, PubMed:24022480). Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine, 8oxoG or abasic sites (PubMed:19074196, PubMed:24191025). {PubMed:16510448, PubMed:19074196, PubMed:20227374, PubMed:20334433, PubMed:24022480, PubMed:24035200, PubMed:24191025, PubMed:24310611}.
Research Areas Enzymes
Application Images
Description DNA polymerase delta antibody [6196] detects DNA polymerase delta protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
DNA polymerase delta stained by DNA polymerase delta antibody [6196] (13910) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Description Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DNA polymerase delta antibody [6196] (13910) diluted at 1:1000.
Additional Information
Handling
Storage This product is stable for at least one (1) year if stored at -20°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a 1:500-1:3,000 dilution. A band of ~124kDa is detected. Detection of DNA polymerase delta in HeLa cell lysate with #13910 diluted 1:1,000

IHC: use at a dilution of 1:200 with antigen retrieval: citrate buffer, pH 6.0, 15 minutes. Detection of DNA polymerase delta in paraffin-embedded human colon carcinoma.

Endusers should determine optimal antibody concentrations for their applications.
References & Data Sheet