Anti-Tau Antibody (57011)

$469.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ELISA,IHC Human, Mouse
SKU: 57011 Categories: , ,
Overview
Product Name Anti-Tau Antibody (57011)
Description Anti-Tau Rabbit Polyclonal Antibody
Target Tau
Species Reactivity Human, Mouse
Applications WB,ELISA,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide derived from the full-length sequence of human Tau
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody recognizes human and mouse Tau.
Target Name Microtubule-associated protein tau
Target ID Tau
Uniprot ID P10636
Alternative Names Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Gene Name MAPT
Sequence Location Cytoplasm, cytosol, Cell membrane, Cytoplasm, cytoskeleton, Cell projection, axon, Cell projection, dendrite, Secreted
Biological Function Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. {PubMed:21985311, PubMed:32961270}.
Research Areas Neuroscience
Application Images
Description Immunohistochemistry analysis using Rabbit Anti-Tau Polyclonal Antibody (57011). Tissue: Brain Cortex. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (57011).
Description Western blot analysis of SH-SY5Y showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (57011). Lane 1: Molecular Weight Ladder (MW). Lane 2: SH-SY5Y. Load: 10 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (57011) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa.
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions ELISA: use at 1-10ug/ml with human Tau on the solid phase.

Immunoblotting: use at 1-5ug/ml. A band of ~45.8kDa is detected.
References & Data Sheet