Awaiting product image

Anti-Excitatory Amino Acid Transporter 1 Antibody (90014)

$520.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ELISA Rat
SKU: 90014 Categories: , ,
Overview
Product Name Anti-Excitatory Amino Acid Transporter 1 Antibody (90014)
Description Anti-Excitatory Amino Acid Transporter 1 Mouse Monoclonal Antibody
Target Excitatory Amino Acid Transporter 1
Species Reactivity Rat
Applications WB,IHC,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 2G7C2
Isotype IgG1
Immunogen Synthetic peptide derived from the C-terminal domain of rat EAAT1. Accession no. P24942.
Properties
Form Lyophilized
Formulation Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
Buffer Formulation Tris Buffer
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody reacts with rat EAAT1 protein. Cross-reactivity with human and mouse EAAT1 is predicted based on sequence homology.
Target Name Excitatory amino acid transporter 1
Target ID Excitatory Amino Acid Transporter 1
Uniprot ID P24942
Alternative Names Glial glutamate transporter, Sodium-dependent glutamate/aspartate transporter 1, GLAST, GLAST-1, Solute carrier family 1 member 3
Gene Name Slc1a3
Sequence Location Cell membrane, Multi-pass membrane protein.
Biological Function Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:1279699, PubMed:8387171). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (By similarity). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity). {UniProtKB:O57321, UniProtKB:P56564, PubMed:1279699, PubMed:8387171}.
Research Areas Neuroscience
Background Excitatory amino-acid transporters (EAATs), also known as glutamate transporters, belong to the family of neurotransmitter transporters. EAATs serve to terminate the excitatory signal by removal of glutamate from the neuronal synapse into neuroglia and neurons. After glutamate is released as the result of an action potential, glutamate transporters quickly remove it from the extracellular space to keep its levels low, thereby terminating the synaptic transmission. The activity of glutamate transporters also allows glutamate to be recycled for repeated release. Glutamate transporters also transport aspartate and are present in virtually all peripheral tissue. They exhibit stereoselectivity for L-glutamate but transport both L- and D-aspartate.
Application Images
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.
Application Instructions ELISA: use at 0.1-2ug/ml

Immunoblotting:

User should use at 1-5ug/ml. A band of ~60kDa is detected.

Immunohistochemistry: Use at 2- 10ug/ml.

These are recommended concentrations.

End user should determine optimal concentrations for their applications.
References & Data Sheet