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Anti-Digoxigenin Antibody (90012)

$520.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ELISA Digoxigenin
SKU: 90012 Categories: , ,
Overview
Product Name Anti-Digoxigenin Antibody (90012)
Description Anti-Digoxigenin Mouse Monoclonal Antibody
Target Digoxigenin
Species Reactivity Digoxigenin
Applications WB,IHC,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 21H8
Isotype IgG1
Immunogen Digoxigenin conjugate.
Properties
Form Lyophilized
Formulation Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
Buffer Formulation Tris Buffer
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody reacts with free and bound digoxigenin.
Target ID Digoxigenin
CAS Number 1672-46-4
PubChem ID 15478
Research Areas Hormones/Proteins
Background Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata (foxgloves). It is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein and is often used as an immunohistochemical marker for in situ hybridization. In this case it is conjugated to a single species of RNA nucleotide triphosphate (typically uridine), which is then incorporated into RNA as it is synthesized.
Application Images
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.
Application Instructions ELISA: use at 0.1-1.0ug/ml

Immunoblotting:

User should use at 2-5ug/ml.

Immunohistochemistry: Use at 1-5ug/ml.

These are recommended concentrations.

End user should determine optimal concentrations for their applications.
References & Data Sheet