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Anti-Calnexin Antibody (90007)

$520.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC Human, Dictyostelium
SKU: 90007 Categories: , ,
Overview
Product Name Anti-Calnexin Antibody (90007)
Description Anti-Calnexin Mouse Monoclonal Antibody
Target Calnexin
Species Reactivity Human, Dictyostelium
Applications WB,IHC
Host Mouse
Clonality Monoclonal
Clone ID C2A1
Isotype IgG1
Immunogen Dictyostelium discoideum AX2 recombinant fragment. Accession no. P27824.
Properties
Form Lyophilized
Formulation Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
Buffer Formulation Tris Buffer
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody reacts with human and Dictyostelium discoideum calnexin.
Target Name Calnexin
Target ID Calnexin
Uniprot ID P27824
Alternative Names IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
Gene Name CANX
Sequence Location Endoplasmic reticulum membrane, Endoplasmic reticulum, Melanosome
Biological Function Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
Research Areas Neuroscience
Background Calnexin is a ~90 kDa integral protein of the endoplasmic reticulum; it is also referred to as IP90, p88 and p90. It consists of a 50kDa N-terminal calcium- binding luminal domain, a single transmembrane helix, and a short acidic cytoplasmic tail. Its positively charged cytosolic residues ensure its retention in the endoplasmic reticulum. Calnexin together with calreticulin play a key role in glycoprotein folding by interacting with folding intermediates via their monoglycosylated glycans. Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor, and B cell surface immunoglobulin.
Application Images
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.
Application Instructions Immunoblotting: use at 1-10ug/ml.

Immunohistochemistry: use at 1-10ug/ml.

These are recommended concentrations.

End user should determine optimal concentrations for their applications.
References & Data Sheet