Anti-Neuroligin-1 Antibody (56551)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF Human, Mouse, Rat
SKU: 56551 Categories: , ,
Overview
Product Name Anti-Neuroligin-1 Antibody (56551)
Description Anti-Neuroligin-1 Mouse Monoclonal Antibody
Target Neuroligin-1
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID S97A-31
Isotype IgG1
Immunogen Fusion protein corresponding to aa 718-843 (cytoplasmic C-terminus) of rat Neuroligin- 1, accession no. Q62765). This sequence is 99
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.1% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat Neuroligin-1. It does not cross- react with other Neuroligins.
Target Name Neuroligin-1
Target ID Neuroligin-1
Uniprot ID Q62765
Alternative Names Neuroligin I
Gene Name Nlgn1
Sequence Location Cell membrane; Single-pass type I membrane protein. Cell junction, synapse, postsynaptic density, Cell junction, synapse, synaptic cleft, Cell junction, synapse, synaptic cell membrane
Biological Function Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Required to maintain wakefulness quality and normal synchrony of cerebral cortex activity during wakefulness and sleep (By similarity). The protein is involved in nervous system development. {UniProtKB:Q99K10, PubMed:15620359, PubMed:9325340}.
Research Areas Neuroscience
Background Neuroligin-1 is a neuronal cell surface protein and member of the type-B carboxylesterase/lipase family. It is a necessary component in the maturation of excitatory synapses, it participates in regulation of synaptic plasticity, and it is important in the development of long- term memory in adult mammalian amygdala. It's participation in cell-cell interactions may occur through assembly of intracellular junctions by binding of beta-neurexins. In nervous system tissue, Neuroligin-1 is observed primarily in neurons and in the spinal cord.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 1 Monoclonal Antibody, Clone S97A-31 (56551). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Neuroligin 1 Monoclonal Antibody (56551) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Neuroligin 1 Antibody (D) Composite.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 1 Monoclonal Antibody, Clone S97A-31 (56551). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Neuroligin 1 Monoclonal Antibody (56551) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Neuroligin 1 Antibody. (D) Composite.
Description Western Blot analysis of Mouse Brain Membrane showing detection of ~120 kDa Neuroligin 1 protein using Mouse Anti-Neuroligin 1 Monoclonal Antibody, Clone S97A-31 (56551). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 1 Monoclonal Antibody (56551) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~120 kDa.
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-5ug/mL. A band of ~120kDa is detected.

Immunofluorescence: use at 10ug/mL.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet