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Anti-Calnexin (NT) Antibody (56414)

$321.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea Pig, Hamster, Pig, Monkey, Rabbit, Sheep
SKU: 56414 Categories: , ,
Overview
Product Name Anti-Calnexin (NT) Antibody (56414)
Description Anti-Calnexin Rabbit Polyclonal Antibody
Target Calnexin (NT)
Species Reactivity Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea Pig, Hamster, Pig, Monkey, Rabbit, Sheep
Applications WB
Host Rabbit
Clonality Polyclonal
Immunogen A 19 amino acid synthetic peptide corresponding to canine calnexin conjugated to KLH.
Properties
Form Liquid
Concentration Lot Specific
Formulation Whole antiserum
Buffer Formulation Whole Antiserum
Format Whole Antiserum
Purification Whole antiserum
Specificity Information
Specificity This antibody recognizes human, mouse, rat, bovine, chicken, dog, guinea pig, hamster, pig, monkey, rabbit, and sheep calnexin.
Target Name Calnexin
Target ID Calnexin (NT)
Uniprot ID P27824
Alternative Names IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
Gene Name CANX
Sequence Location Endoplasmic reticulum membrane, Endoplasmic reticulum, Melanosome
Biological Function Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
Research Areas Neuroscience
Background Calnexin is a ~90 kDa integral protein of the endoplasmic reticulum; it is also referred to as IP90, p88 and p90. It consists of a 50 kDa N-terminal calcium-binding luminal domain, a single transmembrane helix, and a short acidic cytoplasmic tail. Its positively charged cytosolic residues ensure its retention in the endoplasmic reticulum. Calnexin together with calreticulin play a key role in glycoprotein folding by interacting with folding intermediates via their monoglycosylated glycans. Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor, and B cell surface immuno- globulin.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:5,000-1:10,000 dilution. A band of ~90 kDa is detected.

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: HeLa cell lysate.
References & Data Sheet