Anti-p53R2 (NT) Antibody (56224)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Human, Mouse, Rat
SKU: 56224 Categories: , ,
Overview
Product Name Anti-p53R2 (NT) Antibody (56224)
Description Anti-p53R2 (NT) Rabbit Polyclonal Antibody
Target p53R2 (NT)
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Immunogen Peptide corresponding to aa 2-17 of human p53R2.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 0.02% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes an epitope at the N-terminus of human, mouse, and rat p53R2.
Target Name Ribonucleoside-diphosphate reductase subunit M2 B
Target ID p53R2 (NT)
Uniprot ID Q7LG56
Alternative Names EC 1.17.4.1, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein, p53R2
Gene Name RRM2B
Sequence Location Cytoplasm. Nucleus. Note=Translocates from cytoplasm to nucleus in response to DNA damage.
Biological Function Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. {PubMed:10716435, PubMed:11517226, PubMed:11719458}.
Research Areas Cancer research
Application Images
Description Western blot analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Description Immunohistochemistry of p53R2 in human lung tissue with p53R2 antibody at 1 ug/mL.
Description Immunofluorescence of p53R2 in Human Lung tissue with p53R2 antibody at 20 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. A band of ~39kDa is detected

IHC: use at 1ug/mL.

Immunofluorescence: use at 20ugml.
References & Data Sheet