Anti-MTBP Antibody (56222)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF Human, Mouse
SKU: 56222 Categories: , ,
Overview
Product Name Anti-MTBP Antibody (56222)
Description Anti-MTBP Rabbit Polyclonal Antibody
Target MTBP
Species Reactivity Human, Mouse
Applications ELISA,WB,ICC,IF
Host Rabbit
Clonality Polyclonal
Immunogen Peptide corresponding to aa 122-139 of human MTBP. This sequence differs from that of mouse by three amino acids.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human and mouse MTBP which is involved in the p53 network of tumor suppression. p53 transcriptionally activates the MDM2 gene, then the translated MDM2 protein binds to p53 and promotes degradation of p53. MTBP binds to MDM2 and inhibits the modulating effect of MDM2 on p53. MTBP is expressed in a variety of normal tissues.
Target Name Mdm2-binding protein
Target ID MTBP
Uniprot ID Q96DY7
Alternative Names hMTBP
Gene Name MTBP
Biological Function Inhibits cell migration in vitro and suppresses the invasive behavior of tumor cells (By similarity). May play a role in MDM2-dependent p53/TP53 homeostasis in unstressed cells. Inhibits autoubiquitination of MDM2, thereby enhancing MDM2 stability. This promotes MDM2-mediated ubiquitination of p53/TP53 and its subsequent degradation. {PubMed:15632057}.
Research Areas Cancer research
Application Images
Description Western blot analysis of MTBP expression in K562 cell lysate with MTBP antibody at (A) 1 and (B) 2 ug/ml.
Description Immunocytochemistry of MTBP in K562 cells with MTBP antibody at 5 ug/mL.
Description Immunofluorescence of MTBP in K562 cells with MTBP antibody at 20 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-1 ug/mL. In immunoblots, a band of 104 kD is detected.

Positive control: K562 cell lysate.
References & Data Sheet