Anti-Rad51 Antibody (56214)
$503.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Mouse | 100ug | WB,ICC/IF,IHC-P,IP,IHC,PLA | Human |  |
Overview
Product Name Anti-Rad51 Antibody (56214)
Description Anti-Rad51 Mouse Monoclonal Antibody
Target Rad51
Species Reactivity Human
Applications WB,ICC/IF,IHC-P,IP,IHC,PLA
Host Mouse
Clonality Monoclonal
Clone ID 14B4
Isotype IgG2b
Immunogen GST fusion protein corresponding to full-length Rad51 (aa 1-338) of human Rad51 expressed in E. coli.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by affinity on GST
Specificity Information
Specificity This antibody recognizes human Rad51, a 37 kD protein that interacts with BRCA1. This interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact via the BRC repeats located with exon 11 of BRCA2. When these repeats are deleted, the interaction is lost, and cells become hypersensitive to DNA damage caused by methyl methanesulfonate (MMS).
Target Name DNA repair protein RAD51 homolog 1
Target ID Rad51
Uniprot ID Q06609
Alternative Names HsRAD51, hRAD51, RAD51 homolog A
Gene Name RAD51
Sequence Location Nucleus, Cytoplasm, Cytoplasm, perinuclear region. Mitochondrion matrix, Chromosome, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Biological Function Plays an important role in homologous strand exchange, a key step in DNA repair through homologous rPubMed:12205100, PubMed:12442171, PubMed:18417535, PubMed:20231364, PubMed:20348101, PubMed:20413593, PubMed:23509288, PubMed:23754376, PubMed:26253028, PubMed:26681308, PubMed:28575658}.
Research Areas Cancer research
Application Images
Description Immunofluorecent staning of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody (56214).
Cells were pre-extracted with CSK buffer before fixation with 4% PFA.
RAD51 14B4 was used at 1:1000 dultion. DAPI was used to counterstain the nucleus.
Scale bar, 10 mciro-meter.
Cells were pre-extracted with CSK buffer before fixation with 4% PFA.
RAD51 14B4 was used at 1:1000 dultion. DAPI was used to counterstain the nucleus.
Scale bar, 10 mciro-meter.
Description Mouse anti-RAD51 14B4 antibody (56214) was used in IP assay (immunoprecipitation) using HeLa cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40).
Rabbit anti-RAD51 antibody was used for subsequent WB detection of immunoprecipated RAD51.
Whole cell extract was used as input.
Rabbit anti-RAD51 antibody was used for subsequent WB detection of immunoprecipated RAD51.
Whole cell extract was used as input.
Description RAD51 antibody 14B4 (56214) detects RAD51 in nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
RAD51 antibody 14B4 (562148) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample: Paraffin-embedded BT483 xenograft.
RAD51 antibody 14B4 (562148) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Description Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (56214) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Description Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (56214) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Description Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (56214) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Description Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (56214) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Description Rad51 antibody [14B4] detects Rad51 protein at nuclear foci by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rad51 stained by Rad51 antibody [14B4] (56214) diluted at 1:500.Blue: Fluoroshield with DAPI.
Handling
Storage This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting,
Immunoprecipitation: use at 1-5 ug/mL.
Positive control: T24 bladder carcinoma cells and recombinant fusion protein.
Immunoprecipitation: use at 1-5 ug/mL.
Positive control: T24 bladder carcinoma cells and recombinant fusion protein.
References & Data Sheet
References Shinohara, A et al. 1993. Nature Genetics 4: 239-243
Scully, R et al. 1997. Cell 88: 265-275
Chen, PL et al. 1998.
PNAS (USA) 95: 5287-5292.
Scully, R et al. 1997. Cell 88: 265-275
Chen, PL et al. 1998.
PNAS (USA) 95: 5287-5292.
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet