Anti-Rad50 Antibody (56213)

$503.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF,IHC-P,IP,IHC,in vitro Human
SKU: 56213 Categories: , ,
Overview
Product Name Anti-Rad50 Antibody (56213)
Description Anti-Rad50 Mouse Monoclonal Antibody
Target Rad50
Species Reactivity Human
Applications WB,ICC/IF,IHC-P,IP,IHC,in vitro
Host Mouse
Clonality Monoclonal
Clone ID 2C6
Isotype IgG1
Immunogen GST fusion protein corresponding to the complete coding region (aa 1-425) of Rad50 expressed in E. coli.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human Rad50, a 153 kD protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex. Following DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.
Target Name DNA repair protein RAD50
Target ID Rad50
Uniprot ID Q92878
Alternative Names hRAD50, EC 3.6.-.-
Gene Name RAD50
Sequence Location Nucleus, Chromosome, telomere, Chromosome
Biological Function Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA rPubMed:10888888, PubMed:11741547, PubMed:15064416, PubMed:9590181, PubMed:9651580, PubMed:9705271}.
Research Areas Cancer research
Application Images
Description Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad50 protein stained by Rad50 antibody [13B3] (56213) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 um.
Description Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded CAL 27 xenograft.
Rad50 antibody [13B3] (56213) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Description Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded PC-3 xenograft.
Rad50 antibody [13B3] (56213) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Description Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Rad50 stained by Rad50 antibody [13B3] (56213) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Description Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Rad50 stained by Rad50 antibody [13B3] (56213) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Description HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (56213) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting,

Immunoprecipitation: use at 0.1-2 ug/mL.

Positive control: Raji cells.
References & Data Sheet