Mikrogen Diagnostik recomWell Yersina IgM ELISA Kit (4605)

$393.00

Host Quantity Applications Species Reactivity Data Sheet  
96 Tests ELISA Yersina
SKU: 4605 Categories: ,
Overview
Product Name Mikrogen Diagnostik recomWell Yersina IgM ELISA Kit (4605)
Description Enzyme immunoassay with recombinant antigens for the detection of IgG, IgA or IgM antibodies against Yersinia enterocolitica and Yersinia pseudotuberculosis

Product is intended for research use.
Target Human Yersina IgM
Species Reactivity Yersina
Assay Type Indirect ELISA
Applications ELISA
Properties
Background In the past years, Yersinia enterocolitica has become increasingly important as an intestinal bacterium that is pathogenic for human beings. This organism is transmitted orally by contaminated water or food. Typical symptoms of an acute Yersinia infection are diarrhoea, stomach ache, and fever.Complications such as reactive arthritis, erythema nodosum and other rheumatic diseases can occur, especially with HLA-B27 carriers. These patients typically show high and persistent IgA titers against Yersinia antigens.recomWell Yersinia tests are quantitative in vitro tests for the detection and safe identification of antibodies against plasmid-coded virulence proteins. The antigens (YOPs - Yersinia outer membrane proteins) used in these tests are expressed only by strains of Yersinia that are pathogenic for human beings.The use of recombinant antigens guarantees optimum representation of the immundominant YOPs in the absence of cross-reacting proteins. As a second major advantage, the clarification of Yersinia induced reactive arthritis is supproted by separate detection of antibody classes IgG, IgA and IgM.
Sample Type Serum, plasma, whole blood
Assay Resolution See Figure
Components
10X Wash Buffer100 mL
Dilution Buffer125 mL
TMB Substrate12 mL
Stop Slution12 mL
Instuctions for Use1 Each
Evaluation Form1 Each
Microplate Sealing Tape2 Each
Microtiter Plate12 x 8 Wells
Positive Control450 uL
Cut-Off Control450 uL
Negative Control450 uL
Anti-Human IgM Conjugate500 uL
Specificity Information
Target ID Human Yersina IgM
Research Areas Infectious Disease
Application Images
Description Test Procedure and Principle:

Indirect sandwich test. Recombinant antigens are bound to the solid phase.
1. 1st Incubation. Add patient samples diluted 1:101 (sample: 10 µl of serum or plasma), incubate for 1 h at 37 °C.
2. Wash 4 times
3. 2nd Incubation
4. Add peroxidase conjugated anti-human IgG or IgM antibodies (conjugate), incubate for 30 min at 37 °C.
5. Wash 4 times
6. Color reaction
7. Add ready-to-use TMB solution and incubate for 30 min at room temperature. Stop the substrate reaction with H3PO4 and measure the extinction at 450 nm.
Description Test sensitivity specificity.
Additional Information
Handling
Storage Store at 2-8°C.
References & Data Sheet