Anti-HER2 (Phospho-Tyr1248) Antibody (43079)
$366.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Rabbit | 100ul | WB,IHC | Human, Mouse, Rat |  |
Overview
Product Name Anti-HER2 (Phospho-Tyr1248) Antibody (43079)
Description Anti-HER2 (Phospho-Tyr1248) Rabbit Polyclonal Antibody
Target HER2 (Phospho-Tyr1248)
Species Reactivity Human, Mouse, Rat
Applications WB,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of tyrosine 1248 (P-E-Y(p)-L-G) derived from human HER2 and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human, mouse, and rat HER2 only when phosphorylated at tyrosine 1248.Accession no.: P04626, NP_001005862.1
Target Name Receptor tyrosine-protein kinase erbB-2
Target ID HER2 (Phospho-Tyr1248)
Uniprot ID P04626
Gene Name ERBB2
Gene ID 164870
Post Translational Modification HER2 (Phospho-Tyr1248)
Target Phospho-tyr1248
Sequence Location [Isoform 1]: Cell membrane, Cytoplasm, perinuclear region. Nucleus. Note=Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. Also detected in VPS35-positive endosome-to-TGN retrograde vesicles (PubMed:31138794).
Biological Function Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. {PubMed:10358079, PubMed:15380516, PubMed:21555369}.
Research Areas Phosphospecific Antibodies
Background HER2 is a member of the human epidermal growth factor receptor (HER/EGFR/ERBB) family. Amplification or over-expression of this oncogene has been shown to play an important role in the development and progression of certain aggressive types of breast cancer. In recent years HER2 has become an important biomarker and target of therapy for approximately 30% of breast cancer patients.
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~185kDa is detected.
Immunohistochemistry: use at dilution of 1:50-1:100.
Immunofluorescence: use at dilution of 1:100-1:200.
Immunohistochemistry: use at dilution of 1:50-1:100.
Immunofluorescence: use at dilution of 1:100-1:200.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet