Awaiting product image

Anti-Progesterone Receptor (Phospho-Ser190) Antibody (43074)

$366.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC,IF Human
SKU: 43074 Categories: , ,
Overview
Product Name Anti-Progesterone Receptor (Phospho-Ser190) Antibody (43074)
Description Anti-Progesterone Receptor (Phospho-Ser190) Rabbit Polyclonal Antibody
Target Progesterone Receptor (Phospho-Ser190)
Species Reactivity Human
Applications WB,IHC,IF
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of Serine 190 (G-L-S(p)-P-A) derived from human progesterone receptor and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human progesterone receptor only when phosphorylated at serine 190.Accession no.: P06401, NP_000917.3
Target ID Progesterone Receptor (Phospho-Ser190)
Post Translational Modification Progesterone Receptor (Phospho-Ser190)
Target Phospho-ser190
Research Areas Phosphospecific Antibodies
Background Progesterone receptor (PR) is a nuclear hormone receptor of the NR3C class which includes mineralocorticoid, glucocorticoid, and androgen receptors. They exist as homodimers coupled to Hsp90 or HMGB proteins which are shed upon activation. Progesterone receptors signal directly via DNA binding and transcriptional regulation of target genes. They can also signal by binding to other proteins with transcription factors such as NF-kappa B, AP-1, or STAT. Progesterone receptor expression is induced by estrogen, and the human gene encoding the progesterone receptor has been localized to 11q22.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~66kDa is detected.

Immunofluorescence: use at dilution of 1:100-1:200.

Immunohistochemistry: use at dilution of 1:50-1:100.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
References & Data Sheet