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Anti-Ezrin (Phospho-Tyr353) Antibody (43063)

$366.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC Human
SKU: 43063 Categories: ,
Overview
Product Name Anti-Ezrin (Phospho-Tyr353) Antibody (43063)
Description Anti-Ezrin (Phospho-Tyr353) Rabbit Polyclonal Antibody
Target Ezrin (Phospho-Tyr353)
Species Reactivity Human
Applications WB,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from human Ezrin and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human Ezrin only when phosphorylated at tyrosine 353.Accession no.: P15311, NP_001104547.1
Target Name Ezrin
Target ID Ezrin (Phospho-Tyr353)
Uniprot ID P15311
Gene Name EZR
Gene ID 123900
Post Translational Modification Ezrin (Phospho-Tyr353)
Target Phospho-tyr353
Sequence Location Apical cell membrane, Cell projection, Cell projection, microvillus membrane, Cell projection, ruffle membrane, Cytoplasm, cell cortex, Cytoplasm, cytoskeleton, Cell projection, microvillus
Biological Function Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. {PubMed:17881735, PubMed:18270268, PubMed:19111582}.
Research Areas Phosphospecific Antibodies
Background Ezrin (also known as cytovillin or villin-2) is encoded by the EZR gene. The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. It plays a key role in cell surface structure adhesion, migration, and organization. The N-terminal FERM domain strongly binds sodium-hydrogen exchanger regulatory factor (NHERF) proteins (involving long-range allostery). The C-terminal ERM domain binds to actin, phosphatidylinositol(4,5)bis-phosphate (PIP2) and membrane proteins like CD44 and ICAM-2.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~81kDa is detected.

Immunohistochemistry: use at dilution of 1:50- 1:100.

These are recommended working dilutions.

End user should determine optimal dilutions for their application.
References & Data Sheet