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Anti-STAT5A (phospho-Tyr694) Antibody (43048)

$366.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC Human, Mouse, Rat
SKU: 43048 Categories: ,
Overview
Product Name Anti-STAT5A (phospho-Tyr694) Antibody (43048)
Description Anti-STAT5A (phospho-Tyr694) Rabbit Polyclonal Antibody
Target STAT5A (phospho-Tyr694)
Species Reactivity Human, Mouse, Rat
Applications WB,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of tyrosine 694 (D-G-Y(p)-V-K) derived from human STAT5A and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human, mouse, and rat STAT5A only when phosphorylated at tyrosine 694.
Target Name Signal transducer and activator of transcription 5A
Target ID STAT5A (phospho-Tyr694)
Uniprot ID P42229
Gene Name STAT5A
Gene ID 601511
Post Translational Modification STAT5A (phospho-Tyr694)
Target Phospho-tyr694
Sequence Location Cytoplasm, Nucleus
Biological Function Carries out a dual signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. {PubMed:15534001}.
Research Areas Phosphospecific Antibodies
Background Signal transducers and activators of transcription (STATs) are transcription factors that play important roles in signaling pathways induced by many different cytokines and growth factors that regulate cell growth and differentiation, the immune response, antiviral activity, and homeostasis. Their activation and mechanisms of action are examples of direct signaling to the nucleus by extracellular polypeptide ligands without the involvement of second messengers, tyrosine phosphorylation of transcription factors, or transcription factors that make direct contact with membrane-bound receptors and DNA. Since the discovery of STAT 1 and 2, the family has been expanded to seven members.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A band of~90kDa is detected.

Immunohistochemistry: use at dilution of 1:50- 1:100.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
References & Data Sheet