Awaiting product image

Anti-GATA1 (phospho-Ser142) Antibody (43041)

$366.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC Human, Mouse, Rat
SKU: 43041 Categories: ,
Overview
Product Name Anti-GATA1 (phospho-Ser142) Antibody (43041)
Description Anti-GATA1 (phospho-Ser142) Rabbit Polyclonal Antibody
Target GATA1 (phospho-Ser142)
Species Reactivity Human, Mouse, Rat
Applications WB,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of Serine 142 (R-L-S(p)-P-D) derived from human GATA1 and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human, mouse, and rat GATA1 only when phosphorylated at serine 142.
Target Name Erythroid transcription factor
Target ID GATA1 (phospho-Ser142)
Uniprot ID P15976
Gene Name GATA1
Gene ID 305371
Post Translational Modification GATA1 (phospho-Ser142)
Target Phospho-ser142
Sequence Location Nucleus.
Biological Function Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781). {PubMed:22235304, PubMed:24245781}.
Research Areas Phosphospecific Antibodies
Background GATA1 encodes a protein, GATA1, which belongs to the GATA family of transcription factors. This protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~43kDa is detected.

Immunohistochemistry: use at dilution of 1:50-1:100.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications
References & Data Sheet