Anti-Rel (Phospho-Ser503) Antibody (43020)
$366.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Rabbit | 100ul | IF | Human |  |
Overview
Product Name Anti-Rel (Phospho-Ser503) Antibody (43020)
Description Anti-Rel (Phospho-Ser503) Rabbit Polyclonal Antibody
Target Rel (Phospho-Ser503)
Species Reactivity Human
Applications IF
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of Serine 503 (T-S-S(p)-D-S) derived from human Rel and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human Rel only when phosphorylated at serine 503.
Target Name Proto-oncogene c-Rel
Target ID Rel (Phospho-Ser503)
Uniprot ID Q04864
Gene Name REL
Gene ID 164910
Post Translational Modification Rel (Phospho-Ser503)
Target Phospho-ser503
Sequence Location Nucleus
Biological Function Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator.
Research Areas Phosphospecific Antibodies
Background Transcription factor c-Rel is a member of the Rel/NFkappaB family which also includes Rel-A, Rel-B, NFkappaB1, and NFkappaB2. These proteins are related through a highly conserved N-terminal region referred to as the “Rel domain†which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFkappaB inhibitor.
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunofluorescence: use at dilution of 1:100-1:200.
These are recommended working dilutions.
End user should determine optimal dilutions for their applications.
These are recommended working dilutions.
End user should determine optimal dilutions for their applications.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet