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Anti-E-Cadherin Antibody (42027)

$484.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ELISA,IHC,IF,FACS Human
SKU: 42027 Categories: , ,
Overview
Product Name Anti-E-Cadherin Antibody (42027)
Description Anti-Human E-cadherin Mouse Monoclonal Antibody
Target E-Cadherin
Species Reactivity Human
Applications WB,ELISA,IHC,IF,FACS
Host Mouse
Clonality Monoclonal
Clone ID SHE78-7
Isotype IgG2a
Immunogen Human placenta E-Cadherin
Properties
Form Lyophilized
Formulation PBS, pH 7.4, lyophilized.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity This antibody specifically reacts with human E-Cadherin, but does not react with rat E-Cadherin. This antibody inhibits E-Cadherin dependent cell-cell contact.
Target Name Cadherin-1
Target ID E-Cadherin
Uniprot ID P12830
Alternative Names CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD antigen CD324 [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3]
Gene Name CDH1
Sequence Location Cell junction, adherens junction, Cell membrane, Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with α-, beta- and γ-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
Biological Function Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. {PubMed:11976333, PubMed:16417575}.; E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. {PubMed:16417575}.; (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria. {PubMed:10406800, PubMed:17540170, PubMed:8601315}.
Research Areas Cell adhesion
Application Images
Additional Information
Handling
Storage Lyophilized antibody is stable at 4°C for 2 years. Store the reconstituted solution in aliquots at -20°C for one year or at 4°C for 6 months after addition of 0.1% sodium azide by the user. Avoid repeated freeze-thaw cycles.
Dilution Instructions Reconstitute lyophilized antibody in 100ul of distilled water (final concentration 2.0mg/ml).
Application Instructions ELISA: 2ug/ml

Western Blotting

Flow : 2ug/ml, reducing and non-reducing conditions

Flow Cytometry: 1ug/ml.

Immunohistochemistry: 2ug/ml, paraffin- embedded and frozen tissue sections.

Immunofluorescence: 1ug/ml.Cell-Cell Contact Inhibition Assays: 1ug/ml.
References & Data Sheet