Anti-NORE1 Antibody (34010)

$400.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
Rabbit	100ug	WB,ELISA	Human

SKU: 34010 Categories: Antibody Products, Cancer Research Antibodies, Products

Overview

Product Name Anti-NORE1 Antibody (34010)

Description Anti-NORE1 Rabbit Polyclonal Antibody

Target NORE1

Species Reactivity Human

Applications WB,ELISA

Host Rabbit

Clonality Polyclonal

Immunogen Synthetic peptide corresponding to aa residues 313-329 (DNPQKFALFKRIHKDGQ) of human NORE1 conjugated to KLH.

Properties

Form Liquid

Concentration Lot Specific

Formulation PBS, pH 7.4.

Buffer Formulation Phosphate Buffered Saline

Buffer pH pH 7.4

Format Purified

Purification Purified by peptide immuno-affinity chromatography

Specificity This antibody recognizes human, mouse, and rat NORE1 (approx 47 kDa).

Target Name Ras association domain-containing protein 5

Target ID NORE1

Uniprot ID Q8WWW0

Alternative Names New ras effector 1, Regulator for cell adhesion and polarization enriched in lymphoid tissues, RAPL

Gene Name RASSF5

Sequence Location Cytoplasm. Cytoplasm, cytoskeleton. Note=Isoform 2 is mainly located in the perinuclear region of unstimulated primary T-cells. Upon stimulation translocates to the leading edge and colocalizes with ITGAL/LFA-1 in the peripheral zone of the immunological synapse. Isoform 2 is localized to growing microtubules in vascular endothelial cells and is dissociated from microtubules by activated RAP1A.

Biological Function Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association of isoform 2 with activated RAP1A is required for directional movement of endothelial cells during wound healing. May be involved in regulation of Ras apoptotic function. The RASSF5-STK4/MST1 complex may mediate HRAS and KRAS induced apoptosis. {PubMed:12676952, PubMed:12845325, PubMed:15569673}.

Research Areas Cancer research

Background NORE1, first identified by its ability to bind to active Ras, is related in amino acid sequence to the tumor suppressor RASSF1. Expression of NORE1 suppresses colony formation by A549 and G361 cells, inhibits anchorage- independent growth by A549 cells and delays A549 progression through G1 without evidence of increased apoptosis. NORE1 suppresses the growth of tumor cell lines independent of Ras-like proteins or the protein kinases MST1 and 2.

Handling

Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.

Application Instructions Immunoblotting : use at 1:500-1:1,000 dilution.

Positive control: Lysate of 293 T cells expressing NORE1.

References Aoyama Y et al. (2004) Oncogene 23:3426-33.

PMID 15007383

Data Sheet