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Anti-SHPRH Antibody (34009)

$400.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ELISA Human
SKU: 34009 Categories: , ,
Overview
Product Name Anti-SHPRH Antibody (34009)
Description Anti-SHPRH Antibody Rabbit Polyclonal Antibody
Target SHPRH
Species Reactivity Human
Applications WB,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to aa residues 515-535 (DKTKKQAVGSPRKIEKELRKS) of mouse SHPRH conjugated to KLH at the N-terminus.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes mouse SHPRH (approx. 185 kDa) which has 90% sequence homology with human and 95% sequence homology with rat.
Target Name E3 ubiquitin-protein ligase SHPRH
Target ID SHPRH
Uniprot ID Q149N8
Alternative Names EC 2.3.2.27, EC 3.6.4.-, RING-type E3 ubiquitin transferase SHPRH, SNF2, histone-linker, PHD and RING finger domain-containing helicase
Gene Name SHPRH
Biological Function E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'. {PubMed:17108083, PubMed:17130289, PubMed:18719106}.
Research Areas Cancer research
Background A novel gene containing SNF2, PHD- finger, RING-finger, helicase, and linker histone domains termed SHPRH, is a possible candidate for a tumor suppressor gene. These domains are characteristic of several DNA repair proteins, transcription factors, and helicases. Mapping of this gene to 6q24, a region reported to contain a tumor suppressor locus, suggests a role for SHPRH in tumor suppression.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting : use at 1:250 dilution.

Positive control: Mouse brain lysate.
References & Data Sheet
References Sood R. et al. (2003) Genomics 82:153-161.