Anti-ESE-1 Antibody (34005)

$376.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
Rabbit	100ug	WB,ELISA	Human

SKU: 34005 Categories: Antibody Products, Cancer Research Antibodies, Products

Overview

Product Name Anti-ESE-1 Antibody (34005)

Description Anti-ESE-1 Rabbit Polyclonal Antibody

Target ESE-1

Species Reactivity Human

Applications WB,ELISA

Host Rabbit

Clonality Polyclonal

Immunogen N-terminal sequence MAATCEISNIFSNYFS and C-terminal sequence SSGWKEEEVLQSRN of human ESE-1 protein.

Properties

Form Liquid

Concentration Lot Specific

Formulation PBS.

Buffer Formulation Phosphate Buffered Saline

Format Purified

Purification Purified by immunoaffinity chromatography

Specificity This antibody recognizes human ESE-1 (39-41 kD).

Target Name ETS-related transcription factor Elf-3

Target ID ESE-1

Uniprot ID P78545

Alternative Names E74-like factor 3, Epithelial-restricted with serine box, Epithelium-restricted Ets protein ESX, Epithelium-specific Ets transcription factor 1, ESE-1

Gene Name ELF3

Sequence Location Cytoplasm, Nucleus

Biological Function Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development (By similarity). {PubMed:10391676, PubMed:10644990, PubMed:10773884, PubMed:11036073, PubMed:11313868, PubMed:12414801, PubMed:12624109, PubMed:12682075, PubMed:12713734, PubMed:14715662, PubMed:14767472, PubMed:15075319, PubMed:15169914, PubMed:15794755, PubMed:16307850, PubMed:17060315, PubMed:9129154, PubMed:9234700, PubMed:9336459, PubMed:9395241, PubMed:9417054}.

Research Areas Cancer research

Background Epithelium-specific gene regulation plays a critical role during embryogenesis, and the epithelial cell lineage is the first differentiated cell type to appear after fertilization. Until recently, very few epithelial cell-restricted transcription factors had been described. Members of the ets transcription factor/oncogene family have been implicated in human carcinogenesis following the discovery of several distinct chromosomal translocations involving members of the ets family in different types of cancer. ets factors play key roles in transcriptional control of stringently regulated genes, such as genes involved in tissue development, differentiation, angiogenesis, cell cycle control and cell proliferation. ESE-1 is the first member of the ets family that was found to be exclusively expressed and inducible in epithelial cells.

Handling

Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.

Application Instructions Immunoblotting: use at 1:500-1:1,000 dilution

ELISA: use at 1:1,000-1:5,000 dilution.

Data Sheet