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Anti-Thymidine kinase Antibody (34003)

$480.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 200ug WB,ELISA Human
SKU: 34003 Categories: , ,
Overview
Product Name Anti-Thymidine kinase Antibody (34003)
Description Anti-Thymidine Kinase Mouse Monoclonal Antibody
Target Thymidine kinase
Species Reactivity Human
Applications WB,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 3B3.E11
Isotype IgG1
Immunogen 5'-His-tagged recombinant human thymidine kinase.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody specifically recognizes human thymidine kinase.
Target Name Thymidine kinase, cytosolic
Target ID Thymidine kinase
Uniprot ID P04183
Alternative Names EC 2.7.1.21
Gene Name TK1
Sequence Location Cytoplasm.
Research Areas Cancer research
Background Thymidine kinase (TK) belongs to a group of enzymes such as dihydrofolate reductase, thymidylate synthase, and DNA polymerase that are involved in DNA synthesis and precursor production. High levels of these enzymes are present in proliferating cells, and low levels are found in resting cells, due to multiple regulatory mechanisms that ensure exclusive expression of these enzymes in replicating cells. TK is responsible for catalyzing the phosphorylation of thymidine, which functions as a part of the pyrimidine salvage pathway involved in DNA synthesis. The activities of enzymes such as TK are essential for the activation of several chemotherapeutically important nucleoside analogues that are administered as prodrugs.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: antibody at 10 ug/ml will recognize TK in 5, 10, or 20 ug lysates of cells that express TK

ELISA: use antibody at 2-5 ug/ml (optimized for TK on solid phase at 10 ug/ml).
References & Data Sheet