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Anti-Thymidylate synthase Antibody (34002)

$447.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 200ug WB,IP,IHC,ELISA Human
SKU: 34002 Categories: , ,
Overview
Product Name Anti-Thymidylate synthase Antibody (34002)
Description Anti-Thymidylate Synthase Mouse Monoclonal Antibody
Target Thymidylate synthase
Species Reactivity Human
Applications WB,IP,IHC,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 3A7.A11
Isotype IgG1
Immunogen Recombinant human thymidylate synthase produced in E. coli.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity These antibodies specifically recognize human TS (36 kDa). They do not cross-react with rat TS.
Target Name Thymidylate synthase
Target ID Thymidylate synthase
Uniprot ID P04818
Alternative Names TS, TSase, EC 2.1.1.45
Gene Name TYMS
Sequence Location Nucleus, Cytoplasm, Mitochondrion, Mitochondrion matrix, Mitochondrion inner membrane
Biological Function Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway. {PubMed:11278511, PubMed:21876188}.
Research Areas Cancer research
Background Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunohistochemistry: use at 5-10 ug/ml on frozen sections (reactivity on paraffin-embedded sections has not been determined)

Immunoblotting: use at 5-10 ug/ml

ELISA: use at 2-5 ug/ml (optimized for TS on solid phase at 5 ug/ml).

Immunoprecipitation: use #34002 only at 5-10 ug/ml.
References & Data Sheet